Expression Profiles of Tyrosine Kinases in Cultured Follicular Papilla Cells Versus Dermal Fibroblasts
| Autor: | Tung-Tien Sun, Da-Wen Yu, Qiong Cao, Zhijun He |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Keratinocytes
Male medicine.medical_specialty C-Met Receptor Platelet-Derived Growth Factor alpha Molecular Sequence Data Dermatology Follicular cell Biochemistry Gene Expression Regulation Enzymologic chemistry.chemical_compound dermal papilla Growth factor receptor Internal medicine CDC2 Protein Kinase medicine Animals Humans Amino Acid Sequence HGF Rats Wistar Molecular Biology Cells Cultured c-Met Hair follicle biology Kinase Receptor Protein-Tyrosine Kinases Dermis Cell Biology Fibroblasts Protein-Tyrosine Kinases Proto-Oncogene Proteins c-met PDGF Cell biology Rats Endocrinology chemistry Cell culture biology.protein Hepatocyte growth factor Tyrosine kinase Platelet-derived growth factor receptor medicine.drug |
| Zdroj: | Journal of Investigative Dermatology. (2):283-290 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/j.0022-202X.2004.23212.x |
| Popis: | Tyrosine kinases play crucial roles in cell differentiation and proliferation. Using degenerative primed PCR followed by differential display, we analyzed the tyrosine kinase expression profiles of cultured rat follicular papilla (FP) cells versus dermal fibroblasts. We showed that c-met, cdc2, and tec were preferentially expressed in cultured FP cells, whereas alpha-platelet-derived growth factor receptor (alpha-PDGFR) was preferentially expressed in cultured fibroblasts. The cell type specificity of these tyrosine kinases was confirmed by semi-quantitative RT-PCR using both rat and human cultured cells. Consistent with these results, hepatocyte growth factor preferentially stimulated the growth of rat FP cells, whereas PDGF-AA preferentially stimulated rat fibroblasts. High concentrations of some these kinases are also found in the follicular matrix keratinocytes as revealed by in situ hybridization. The expression of specific tyrosine kinases in FP and matrix cells may play roles in regulating hair growth and cycling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|