Quantification of telomerase activity by direct scintillation counting

Autor:	Lyubomir T. Vassilev, Keh-Ming Pan, Sonja Kazmer
Rok vydání:	1999
Předmět:	Human telomerase Telomerase biology Chemistry Biophysics Biotin Biochemistry Enzyme assay Reaction product Cell Line Magnetics Biotinylation Scintillation counter biology.protein Humans Scintillation Counting Streptavidin Primer (molecular biology) Phosphorus Radioisotopes HeLa Cells
Zdroj:	Journal of biochemical and biophysical methods. 40(3)
ISSN:	0165-022X
Popis:	An improved telomerase assay was developed that allows direct quantification of the enzyme activity by scintillation counting of the labeled telomerase product. The assay measures the incorporation of 32 P-dGTP into telomeric repeats synthesized at the 3′ end of a biotinylated primer. Telomerase reaction product is separated from the reaction mix by streptavidin-coated magnetic beads and counted. The assay can be used for quantitative studies of human telomerase and its inhibitors.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::682afffeda5be48dfd0c6c4713af7248 https://pubmed.ncbi.nlm.nih.gov/10487165 Zobrazit plný text záznamu Full Text from ScienceDirect