Identification of conserved antigens for early serodiagnosis of relapsing fever Borrelia
| Autor: | Sandra J. Raffel, Carl H. Hammer, Mary Ann Robinson, Ming Zhao, Merry E. Schrumpf, Tom G. Schwan, Job E. Lopez, Stephen F. Porcella |
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| Rok vydání: | 2009 |
| Předmět: |
Molecular Sequence Data
Hypothetical protein Microbiology Microbial Pathogenicity Mice Bacterial Proteins Tandem Mass Spectrometry Borrelia Animals Humans Electrophoresis Gel Two-Dimensional Serologic Tests Amino Acid Sequence Conserved Sequence Glycerophosphodiester phosphodiesterase Antigens Bacterial biology Phosphoric Diester Hydrolases Relapsing Fever biology.organism_classification Virology Recombinant Proteins Ornithodoros hermsi Immunoglobulin M Membrane protein biology.protein Borrelia hermsii Borrelia recurrentis |
| Zdroj: | Microbiology |
| ISSN: | 1465-2080 1350-0872 |
| Popis: | Borrelia hermsii is a blood-borne pathogen transmitted by the argasid tick Ornithodoros hermsi. Since spirochaete clearance in mice is associated with an IgM-mediated response, an immunoproteomic analysis was used to identify proteins reactive with IgM. We report that IgM from both mice and human patients infected with B. hermsii not only reacted with the previously identified variable membrane proteins but also identified candidate antigens including heat-shock proteins, an adhesin protein, ABC transporter proteins, flagellar proteins, housekeeping proteins, an immune evasion protein, and proteins with unknown function. Furthermore, IgM reactivity to recombinant glycerophosphodiester phosphodiesterase was detected during early spirochaete infection and prior to a detectable IgG response. Lastly, a conserved hypothetical protein was produced in Escherichia coli and tested with immune serum against B. hermsii and Borrelia recurrentis. These results identify a much larger set of immunoreactive proteins, and could help in the early serodiagnosis of this tick-borne infection. |
| Databáze: | OpenAIRE |
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