Is there evidence for excessive free radical production in vivo during ultrasound-assisted liposuction?
| Autor: | Eberhard Wieland, Catherine Hertl, Rolf-Rudiger Olbrisch, Jürgen Herr, Holger Hofheinz |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Free Radicals medicine.medical_treatment Ultrasound assisted Thiobarbituric Acid Reactive Substances Standard procedure Molecular level Lipectomy In vivo Malondialdehyde medicine Humans Ultrasonics Adverse effect business.industry Ultrasound Middle Aged medicine.disease Surgery Adipose Tissue Liposuction Female Lipid Peroxidation Lipodystrophy business |
| Zdroj: | Plastic and reconstructive surgery. 111(1) |
| ISSN: | 0032-1052 |
| Popis: | The surgical technique of ultrasound-assisted liposuction has become a standard procedure for the treatment of lipodystrophy. However, little is known about the impact of this therapy on fatty tissue on the molecular level. There are concerns about possible adverse effects related to the high-intensity ultrasound energy, because in vitro studies have shown a substantial generation of free radicals. In this study, the authors investigated whether ultrasound waves can create an excessive free radical production in vivo by measuring lipid peroxidation products in the form of malondialdehyde equivalents. For this purpose, the thiobarbituric acid-reactive substances (TBARS) assay was chosen. In this test, malondialdehyde, a major product of lipid peroxidation, reacts with thiobarbituric acid to produce a pink adduct that can be measured spectrophotometrically. The authors determined oxidation products in 28 aspirates of 17 treated patients before ultrasound-assisted liposuction (0 minutes) to establish a baseline concentration and at 2, 5, and 10 minutes after the treatment was begun. Median malondialdehyde concentration of the control group (conventional liposuction, 0 minutes) was 3.40 nmol of malondialdehyde per gram of adipose tissue. Median concentrations after 2, 5, and 10 minutes of ultrasound-assisted liposuction were 7.45 (n = 28), 8.84 (n = 21), and 4.07 (n = 8) nmol malondialdehyde per gram adipose tissue, respectively. The differences were not statistically significant. The data suggest that there is no excessive formation of lipid oxidation products in response to free radicals. The antioxidative capacity of adipose tissue does not seem to be overwhelmed by the standard application regimen of ultrasound-assisted liposuction. |
| Databáze: | OpenAIRE |
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