Osteogenic differentiation characteristics of hip joint capsule fibroblasts obtained from patients with ankylosing spondylitis
| Autor: | Hui-ying Liang, Nan Jiang, Ben-Yong Liu, Xing-hua Feng, Ying-Yan Zhou, Hong-xiao Liu |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.diagnostic_test Ossification General Medicine Biology Bone morphogenetic protein Phenotype Hedgehog signaling pathway Flow cytometry 03 medical and health sciences Tissue culture 030104 developmental biology 0302 clinical medicine Real-time polymerase chain reaction medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer research medicine Original Article medicine.symptom Fibroblast |
| Zdroj: | Ann Transl Med |
| ISSN: | 2305-5847 2305-5839 |
| DOI: | 10.21037/atm-20-7817 |
| Popis: | Background Autoimmune disease are fairly common and one that has an excessive degree of disability is Ankylosing spondylitis (AS). As the main cells in connective tissues, fibroblasts may play important roles in AS ossification. The conducted research aims to establish the osteogenic disparity characteristics of fibroblasts cultured in vitro, obtained via AS patients hip joint capsule, as well as investigating the pathological osteogenic molecular workings of AS. Methods AS patients hip joint capsules were acquired and fracture patients as the control with the finite fibroblast line were established by using tissue culture method. AS fibroblast proliferation, cycle and apoptosis, expression of osteogenic marker genes, osteogenic phenotypes, and the activation degree of the bone morphogenetic protein (BMP)/Smads signalling pathway were detected by flow cytometry, western blotting and real-time fluorescent quantitative polymerase chain reaction. Results Proliferative activity in AS fibroblasts were abnormally high, and the apoptotic rate decreased. Compared with normal fibroblasts, the mRNA expression of osteogenic marker genes, expression of osteogenic phenotypes, protein expression of core-binding factor a1 (Cbfa1), Smad1, Smad4, Smad5, phosphorylated (p) Smad1, and pSmad5 in AS fibroblasts were higher; however, the expression of Smad6 was lower. Moreover, recombinant human bone morphogenetic protein-2(rhBMP-2) stimulated Cbfa1 expression by normal and AS fibroblasts through the BMP/Smads signalling pathway. Conclusions The fibroblasts of hip joint capsules in patients with AS cultured in vitro have biologic characteristics of osteogenic differentiation and may be important target cells of AS ossification. The Activated BMP/Smads signalling pathway could potentially be a mechanism relating to fibroblasts differentiating into osteoblasts and an ossification mechanism for AS. |
| Databáze: | OpenAIRE |
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