Brain norepinephrine identified by mass spectrometry is associated with reproductive status of females of the linden bug Pyrrhocoris apterus
| Autor: | Hana Vaneckova, Daniela Chvalová, Magdalena Hodkova, Lenka Zdechovanova |
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| Rok vydání: | 2014 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization medicine.medical_specialty Physiology Dopamine media_common.quotation_subject Diapause Biochemistry High-performance liquid chromatography Heteroptera Norepinephrine Catecholamines Internal medicine medicine Animals Tilia Octopamine Molecular Biology media_common photoperiodism biology Reproduction Brain Pyrrhocoris biology.organism_classification Endocrinology Female Octopamine (neurotransmitter) Seasons Chromatography Liquid medicine.drug |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 168:70-75 |
| ISSN: | 1096-4959 |
| DOI: | 10.1016/j.cbpb.2013.11.003 |
| Popis: | Several biogenic amines, including controversial presence of norepinephrine (NE), were identified by the high performance liquid chromatography equipped with electrospray ionisation mass spectrometry in brain complexes of adult females of Pyrrhocoris apterus. Quantitative analysis was performed by the high performance liquid chromatography coupled to electrochemical detector. Levels of NE, dopamine (DA), octopamine (OA) and 5-hydroxytryptamine (5-HT) in brain complexes were measured in reproductive vs. diapause females. In field collected samples, levels of NE and DA were significantly higher in reproductive (May) than in non-reproductive (Sep, Oct, Feb) females. In laboratory females, NE is higher in long day photoperiod (reproduction) than in short day photoperiod (diapause) already from the first week of the adult age, while DA shows differences between the two contrasting photoperiods only from the second week of the adult age. No association between reproductive status and levels of OA and 5-HT was found. |
| Databáze: | OpenAIRE |
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