Identification of peripheral oxytocin‐expressing cells using systemically applied cell‐type specific adeno‐associated viral vector
| Autor: | Luis Paiva, Mike Ludwig, Andrew Allchorne, Valery Grinevich, Maja Lozic |
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| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Endocrinology Diabetes and Metabolism Neuropeptide 030209 endocrinology & metabolism Biology Oxytocin Enteric Nervous System Viral vector Rats Sprague-Dawley 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Endocrinology Posterior pituitary Internal medicine Lactation Testis medicine Animals Promoter Regions Genetic Pancreas Neurons Gastrointestinal tract Endocrine and Autonomic Systems Rats Gastrointestinal Tract medicine.anatomical_structure Enteric nervous system hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of Neuroendocrinology. 33 |
| ISSN: | 1365-2826 0953-8194 |
| Popis: | Oxytocin is primarily synthesised in the brain and is widely known for its role in lactation and parturition after being released into the blood from the posterior pituitary gland. Nevertheless, peripheral tissues have also been reported to express oxytocin. Using systemic injection of a recombinant adeno-associated virus vector, we investigated the expression of the green fluorescent protein Venus under the control of the oxytocin promoter in the gastrointestinal tract, pancreas and testes of adult rats. Here, we confirm that the vector infects oxytocin neurones of the enteric nervous system in ganglia of the myenteric and submucosal plexuses. Venus was detected in 25%-60% of the ganglia in the myenteric and submucosal plexuses identified by co-staining with the neuronal marker PGP9.5. Oxytocin expression was also detected in the islets of Langerhans in the pancreas and the Leydig cells of the testes. Our data illustrate that peripheral administration of the viral vector represents a powerful method for selectively labelling oxytocin-producing cells outside the brain. |
| Databáze: | OpenAIRE |
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