Persistent Helicobacter pullorum colonization in C57BL/6NTac mice: a new mouse model for an emerging zoonosis
| Autor: | James G. Fox, Zhongming Ge, Laura D. Cacioppo, Samuel R. Boutin, Nicola Parry, Michelle L. Turk, Zeli Shen, Mark T. Whary, Hilton J. Klein |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Time Factors Helicobacter pullorum Colon animal diseases Enzyme-Linked Immunosorbent Assay Real-Time Polymerase Chain Reaction Microbiology Helicobacter Infections Serology Caecum Feces Mice Cecum Zoonoses medicine Animals Humans Pathogen Mice Inbred C3H biology Models of Infection Hepatobiliary disease Outbreak General Medicine biology.organism_classification Antibodies Bacterial Virology Rats Mice Inbred C57BL Disease Models Animal Chronic infection medicine.anatomical_structure Immunoglobulin G Chronic Disease Female Chickens |
| Zdroj: | Journal of Medical Microbiology. 61:720-728 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Helicobacter pullorum, an enterohepatic Helicobacter species, is associated with gastroenteritis and hepatobiliary disease in humans and chickens. Recently, a novel H. pullorum outbreak in barrier-maintained rats and mice was described. In this study, persistence of infection and serological responses were further evaluated in H. pullorum-infected female C57BL/6NTac and C3H/HeNTac mice obtained from the barrier outbreak. C57BL/6NTac mice (n=36) aged 10-58 weeks were confirmed to be chronically infected with H. pullorum by PCR or culture of caecum, colon and faeces, with no evidence of hepatic infection; two of three C3H/HeNTac mice cleared H. pullorum infection by 26 weeks of age. A quantitative PCR (qPCR) assay based on the cdtB gene specific to H. pullorum demonstrated that colonization was high in the caecum and colon at 10(4)-10(6) c.f.u. equivalents per µg host DNA, and decreased by several logs from 32 to 58 weeks of age. Infected mice were seropositive by ELISA, and H. pullorum-specific IgG levels decreased as colonization was lost over time in selected mice. Consistent with the lack of pathology associated with chronic infection of C57BL/6 mice with other murine enteric helicobacters, C57BL/6NTac and C3H/HeNTac mice infected with H. pullorum did not develop gross or histological lesions of the liver or gastrointestinal tract. The cdtB-based qPCR assay can be used in screening animals, food sources and environmental samples for H. pullorum, as this food-borne pathogen has zoonotic potential. These findings will also allow future studies in murine models to dissect potential pathogenic mechanisms for this emerging pathogen. |
| Databáze: | OpenAIRE |
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