Epidemiology of Aichi virus in fecal samples from outpatients with acute gastroenteritis in Northwestern Spain
| Autor: | Miguel F. Varela, Jesús L. Romalde, Enrique Rivadulla |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Diarrhea Male Kobuvirus Adolescent Genotype Genotyping Techniques animal diseases 030106 microbiology Biology Polymerase Chain Reaction Virus 03 medical and health sciences Feces Young Adult 0302 clinical medicine Virology Outpatients Humans 030212 general & internal medicine Child Genotyping Phylogeny Subclinical infection Aged Aged 80 and over Picornaviridae Infections Coinfection Infant Newborn virus diseases Outbreak Infant Sapovirus Sequence Analysis DNA Middle Aged biology.organism_classification Gastroenteritis Infectious Diseases Spain Child Preschool Female Aichi virus |
| Zdroj: | Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 118 |
| ISSN: | 1873-5967 |
| Popis: | Background In recent years, Aichi virus (AiV) has been involved in acute viral gastroenteritis outbreaks. However, the common pathogenesis of AiV releases more in subclinical infections underestimating the impact of AiV in human health. Objectives The present study describes the presence and genetic diversity of AiV in patients with gastroenteritis in Northwestern Spain. Study design: A total of 2667 stool samples, obtained between July 2010 and June 2011, from diarrheic outpatients were studied for detection and molecular characterization of AiV using PCR techniques followed by sequencing and phylogenetic analyses. Results The virus was detected in 124 (5.0%) of the samples among all age groups. Coinfections were also detected, from the 124 positive samples, 72 (58.1%) were positive only for AiV, whereas mixed contaminations with Norovirus genogroup I or genogroup II, Sapovirus, or other enteric pathogens were detected in 52 (41.9%) samples. A total of 70 positive samples could be genotyped, being characterized as genotype A (58.6%) or B (41.4%). AiV was detected from August to April, being the highest number of AiV positive samples detected during autumn and winter seasons. Conclusions This survey remarks the importance of emerging enteric viruses in patients who require medical assistance, and offers more information about the real importance of AiV as gastroenteritis agent. |
| Databáze: | OpenAIRE |
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