Comparative study on retention behaviour and enantioresolution of basic and neutral structurally unrelated compounds with cellulose-based chiral stationary phases in reversed phase liquid chromatography-mass spectrometry conditions

Autor:	Mireia Pérez-Baeza, Laura Escuder-Gilabert, Yolanda Martín-Biosca, Salvador Sagrado, María José Medina-Hernández
Rok vydání:	2022
Předmět:	Chromatography Reverse-Phase Organic Chemistry Phenylcarbamates Stereoisomerism General Medicine Cellulose Biochemistry Chromatography High Pressure Liquid Mass Spectrometry Analytical Chemistry
Zdroj:	Journal of Chromatography A. 1673:463073
ISSN:	0021-9673
DOI:	10.1016/j.chroma.2022.463073
Popis:	A comparative study on the retention behaviour and enantioresolution of 54 structurally unrelated neutral and basic compounds using five commercial cellulose-based chiral stationary phases (CSPs) and hydro-organic mobile phases compatible with MS detection is performed. Four phenylcarbamate-type cellulose CSPs (cellulose tris(3,5-dimethylphenylcarbamate), Cell1; cellulose tris(3-chloro-4-methylphenylcarbamate), Cell2; cellulose tris(4-chloro-3-methylphenylcarbamate), Cell4 and cellulose tris(3,5- dichlorophenylcarbamate), Cell5) and one benzoate-type cellulose CSP (cellulose tris(4-methylbenzoate), Cell3) are assayed. Mobile phases consist of binary mixtures of methanol (30-90% MeOH) or acetonitrile (10-98% ACN) with 5 mM ammonium bicarbonate (pH = 8.0). The existence of reversed phase (RPLC) and hydrophilic interaction liquid chromatography (HILIC) retention behaviour domains is explored. In MeOH/H
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::681c5b513643920e0e778c891e7cd428 https://doi.org/10.1016/j.chroma.2022.463073 Zobrazit plný text záznamu Full Text from ScienceDirect