Nuclear DNA content, an adjunct to p53 and Ki-67 as a marker of resistance to radiation therapy in oral cavity and pharyngeal squamous cell carcinoma
| Autor: | Bernard Têtu, Isabelle Bairati, André Fortin, Roger A. Monteil, Renald Morency, Hélène Raybaud |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty medicine.medical_treatment Brachytherapy Radiation Tolerance Radioresistance Biomarkers Tumor Rosaniline Dyes medicine Humans Coloring Agents Aged Neoplasm Staging Aged 80 and over Mouth neoplasm Ploidies biology business.industry Head and neck cancer Pharyngeal Neoplasms Radiotherapy Dosage DNA Neoplasm Middle Aged Prognosis medicine.disease Diploidy Immunohistochemistry Radiation therapy Ki-67 Antigen Epidermoid carcinoma Otorhinolaryngology Ki-67 Carcinoma Squamous Cell biology.protein Female Mouth Neoplasms Pharyngeal Squamous Cell Carcinoma Surgery Neoplasm Recurrence Local Tumor Suppressor Protein p53 Oral Surgery business Cell Division Follow-Up Studies Forecasting |
| Zdroj: | International Journal of Oral and Maxillofacial Surgery. 29:36-41 |
| ISSN: | 1399-0020 0901-5027 |
| DOI: | 10.1034/j.1399-0020.2000.290109.x |
| Popis: | Factors of prognosis and radioresistance in oral cavity and pharyngeal squamous cell carcinoma (OCPSCC) are limited. In the present study, the usefulness of tumor DNA content in predicting radioresistance in patients with OCPSCC has been investigated. Radioresistance has been defined as local recurrence or tumor persistence after radiation therapy. DNA-ploidy analysis was performed by static cytometry on smears of cell suspensions obtained from formalin-fixed paraffin-embedded material and stained with Feulgen. DNA-ploidy was correlated with the proliferation rate (Ki-67) and p53 protein accumulation obtained by immunohistochemistry. The follow-up of patients ranged from 8 to 62 months. Radioresistance was more common in non-diploid tumors; 14/28 (50%) non-diploid tumors recurred, whereas only 3 (10.7%) out of 28 diploid tumors had local failure (P=0.0019). Proliferation rate and p53 accumulation, evaluated by immunohistochemistry, also added prognostic information. Twelve out of 14 failures were from non-diploid tumors with a low proliferation rate (Ki-67 |
| Databáze: | OpenAIRE |
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