The StLAC2 gene is required for cell wall integrity, DHN-melanin synthesis and the pathogenicity of Setosphaeria turcica
| Autor: | Keke Cao, Zhiyan Cao, Zhimin Hao, Hui Jia, Zhiyong Li, N. Liu, Jingao Dong, Dongqing Dai, Jinpin Zang, Shuangxin Ma, Shouqin Gu, Chuan Meng |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genes Fungal Mutant Morphogenesis Naphthols Gene Mutant Biology Zea mays Microbiology Cell wall 03 medical and health sciences Ascomycota Cell Wall Genetics Gene Ecology Evolution Behavior and Systematics Plant Diseases Melanins Laccase Setosphaeria turcica Virulence biology.organism_classification Cell biology 030104 developmental biology Infectious Diseases Gene Deletion Intracellular |
| Zdroj: | Fungal Biology. 121:589-601 |
| ISSN: | 1878-6146 |
| Popis: | Laccases are blue multicopper oxidases, play important roles in various biological processes. These processes include fungal dihydroxynaphthalene (DHN)-melanin biosynthesis and pathogenicity, cellular growth, morphogenesis, and differentiation. This study investigated functions of the laccase gene StLAC2 in Setosphaeria turcica. The Δlac2 mutant colony color was distinct from that of the S. turcica wild-type (WT) isolate, and the mutants exhibited defective conidial formation. In contrast to the WT, the mutants exhibited a lighter color on the 2, 2-azino-di-[3-ethylbenzo-thia-zolin-sulphonate] (ABTS) plates, and the intracellular laccase activity was lower. Notably, StLAC2 gene loss correlated with decreased DHN-melanin biosynthesis and affected the integrity of the cell wall, where the StLAC2 gene mutants showed thinner, more transparent walls with a higher number of mitochondria than the WT. The Δlac2 mutants also lost their pathogenicity in maize. The results indicated that the StLAC2 gene involved in cell wall integrity, melanin biosynthesis and appressorial and conidial formation. |
| Databáze: | OpenAIRE |
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