Photon-counting device compatible with conventional flow cytometric data acquisition electronics
| Autor: | Alex Florians, Juleon M. Schins, Jan Greve, A. Agronskaia, Bart G. de Grooth, Kees van der Werf |
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| Rok vydání: | 1998 |
| Předmět: |
Physics
Time delay and integration Photomultiplier Photon Physics::Instrumentation and Detectors business.industry Single molecule measurements Photon-counting Detector Biophysics Photodetector Cell Biology Hematology Photon counting Pathology and Forensic Medicine Endocrinology Optics Analog signal Data acquisition Flow cytometry business |
| Zdroj: | Cytometry, 32(3), 255-259. Wiley |
| ISSN: | 1097-0320 0196-4763 |
| DOI: | 10.1002/(sici)1097-0320(19980701)32:3<255::aid-cyto12>3.0.co;2-m |
| Popis: | We present an electronic scheme that enables us to use a photon-counting device (photomultiplier or avalanche photodetector) for measuring extremely weak signals in a flow cytometer. It can be used as a sole detector, or in combination with other (conventional) detectors using the data acquisition hardware of a conventional flow cytometer. The essential principle is that photon-counting pulses are converted to an analogue signal that is continuously proportional to the number of detected photons during the last integration time. The integration time should be approximately equal to the time an object is illuminated in the flow chamber. In this way, the photon burst due to real events is measured correctly and discriminated from the background pulses (fluorescence and Raman). The use of this scheme for the measurement of single DNA molecules is illustrated. |
| Databáze: | OpenAIRE |
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