Correction: Corrigendum: Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells
| Autor: | Henry Yang, Kenneth R. Chien, Kristina Buac, Hao Wu, Lie Bu, Bing Lim, Shi-Yan Ng, Joo-Hye C. Park, Massimo Nichane, Xiaobing He, Ulrika Felldin, Ronald A. Li, Boon Seng Soh, Jiejia Xu, Kylie S. Foo, Xiaojun Lian |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Science Human Embryonic Stem Cells LIM-Homeodomain Proteins Cardiovascular research General Physics and Astronomy Library science 02 engineering and technology Biology Cardiovascular System Regenerative medicine General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences Mice Inbred NOD Animals Humans Myocytes Cardiac General hospital Progenitor cell Cell Proliferation Multidisciplinary Endothelin-1 Cell Differentiation General Chemistry 021001 nanoscience & nanotechnology Corrigenda Embryonic stem cell Endothelin 1 Cell and molecular biology 030104 developmental biology Stem cell 0210 nano-technology Transcription Factors |
| Zdroj: | Nature Communications, Vol 7, Iss 1, Pp 1-1 (2016) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Coronary arteriogenesis is a central step in cardiogenesis, requiring coordinated generation and integration of endothelial cell and vascular smooth muscle cells. At present, it is unclear whether the cell fate programme of cardiac progenitors to generate complex muscular or vascular structures is entirely cell autonomous. Here we demonstrate the intrinsic ability of vascular progenitors to develop and self-organize into cardiac tissues by clonally isolating and expanding second heart field cardiovascular progenitors using WNT3A and endothelin-1 (EDN1) human recombinant proteins. Progenitor clones undergo long-term expansion and differentiate primarily into endothelial and smooth muscle cell lineages in vitro, and contribute extensively to coronary-like vessels in vivo, forming a functional human-mouse chimeric circulatory system. Our study identifies EDN1 as a key factor towards the generation and clonal derivation of ISL1(+) vascular intermediates, and demonstrates the intrinsic cell-autonomous nature of these progenitors to differentiate and self-organize into functional vasculatures in vivo. |
| Databáze: | OpenAIRE |
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