Binding of the periplakin linker requires vimentin acidic residues D176 and E187
| Autor: | Michael Overduin, Timothy J. Knowles, Penelope Rodriguez-Zamora, Elena Odintsova, Jitendra Kumar, Mark Jeeves, Pooja Sridhar, Martyn Chidgey, Catharine A. Trieber, Claudia Fogl, Fiyaz Mohammed, Caezar Al-Jassar, Tzu-Han Huang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Models Molecular Amino Acids Acidic Intermediate filament cytoskeleton Intermediate Filaments Mutation Missense Medicine (miscellaneous) Glutamic Acid Vimentin General Biochemistry Genetics and Molecular Biology Article Cell membrane 03 medical and health sciences 0302 clinical medicine Protein structure medicine Humans Protein Interaction Domains and Motifs Amino Acid Sequence Protein Structure Quaternary Periplakin lcsh:QH301-705.5 Plakin Aspartic Acid biology Desmoplakin Chemistry Plakins 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) biology.protein Biophysics Molecular modelling General Agricultural and Biological Sciences Linker 030217 neurology & neurosurgery HeLa Cells Protein Binding |
| Zdroj: | Communications Biology Communications Biology, Vol 3, Iss 1, Pp 1-13 (2020) |
| ISSN: | 2399-3642 |
| Popis: | Plakin proteins form connections that link the cell membrane to the intermediate filament cytoskeleton. Their interactions are mediated by a highly conserved linker domain through an unresolved mechanism. Here analysis of the human periplakin linker domain structure reveals a bi-lobed module transected by an electropositive groove. Key basic residues within the periplakin groove are vital for co-localization with vimentin in human cells and compromise direct binding which also requires acidic residues D176 and E187 in vimentin. We propose a model whereby basic periplakin linker domain residues recognize acidic vimentin side chains and form a complementary binding groove. The model is shared amongst diverse linker domains and can be used to investigate the effects of pathogenic mutations in the desmoplakin linker associated with arrhythmogenic right ventricular cardiomyopathy. Linker modules either act solely or collaborate with adjacent plakin repeat domains to create strong and adaptable tethering within epithelia and cardiac muscle. Odinstova, Mohammed, Trieber et al. use structure-based mutagenesis to identify key residues in human periplakin and desmoplakin linker modules required for co-localization with vimentin intermediate filaments. They show that vimentin D176 and E187 are required for direct binding with the periplakin linker. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|