Protective Effect of Gamma-Hydroxybutyrate and Nicotinamide on Low-Dose Streptozotocin-Induced Diabetes in Mice
| Autor: | R. Guintrand-Hugret, M. Reynier, A. Orsetti, O. Bouix |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Blood Glucose
Male Niacinamide Glycosuria medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism Clinical Biochemistry Biochemistry Streptozocin Diabetes Mellitus Experimental Islets of Langerhans Mice chemistry.chemical_compound Endocrinology Internal medicine Diabetes mellitus Insulin Secretion medicine Animals Insulin Rats Wistar geography geography.geographical_feature_category Nicotinamide Pancreatic islets Biochemistry (medical) nutritional and metabolic diseases Drug Synergism Gamma hydroxybutyrate General Medicine Streptozotocin medicine.disease Islet Rats medicine.anatomical_structure chemistry medicine.symptom Beta cell Sodium Oxybate medicine.drug |
| Zdroj: | Hormone and Metabolic Research. 27:216-220 |
| ISSN: | 1439-4286 0018-5043 |
| DOI: | 10.1055/s-2007-979943 |
| Popis: | The purpose of this study was to investigate the effect of a treatment by gamma-hydroxybutyrate (GHB) and nicotinamide (NA) on low-dose streptozotocin (STZ)-induced diabetes in mice. Mean plasma glucose level was significantly elevated in mice given STZ by day 12 after the first STZ injection compared to controls (15.0 +/- 4.7 vs 8.0 +/- 1.6 mmol/l, p < 0.001) and 100% of the animals were severely diabetic by day 18. Plasma glucose levels remained in the normal range and no diabetic values were found in mice treated with combined treatment by GHB and NA for 25 days. However, hyperglycemia and glycosuria appeared within one week after discontinuation of the treatment. Treatment by either GHB or NA alone had only a slight and transient effect in preventing hyperglycemia. In vitro experiment on isolated pancreatic islets demonstrated that STZ-induced loss of insulin response to glucose was also counteracted by incubation with GHB and NA (Peak insulin response to 16.4 mM glucose: 0.69 +/- 0.31 vs 3.03 +/- 0.67 microU/islet/min), but not by GHB or NA alone. These results indicate that GHB and NA have complementary effects in preventing STZ-induced beta cell damage both in vivo and in vitro. This should be taken into account for future preventive strategies in human insulin-dependent diabetes mellitus. |
| Databáze: | OpenAIRE |
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