[Effects of rhubarb aglycone on matrix metalloproteinase in cerebral ischemic tissue in rats with bone marrow mesenchymal stem cell transplantation]
| Autor: | Yue-wu Zhao, Jie Sun, Jian-sheng Li, Jing-xia Liu, Ning Li, Xiao-yan Guo, Jing Su |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Collagen Type IV
Male medicine.medical_specialty Matrix metalloproteinase Biology Pharmacology Mesenchymal Stem Cell Transplantation Brain Ischemia Rats Sprague-Dawley chemistry.chemical_compound Type IV collagen Random Allocation medicine Animals Rheum Tissue Inhibitor of Metalloproteinase-1 Mesenchymal stem cell Infarction Middle Cerebral Artery Surgery Rats Transplantation Aglycone medicine.anatomical_structure Complementary and alternative medicine chemistry Matrix Metalloproteinase 9 Reperfusion Injury Immunohistochemistry Basal lamina Female Bone marrow Drugs Chinese Herbal Phytotherapy |
| Zdroj: | Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine. 6(8) |
| ISSN: | 1672-1977 |
| Popis: | OBJECTIVE To observe the effects of rhubarb aglycone on pathological changes and activity of matrix metalloproteinase in cerebral ischemic tissue in rats with bone marrow mesenchymal stem cell (BMSC) transplantation, and to explore the action mechanisms of rhubarb aglycone in protecting against brain micrangium injury in rats. METHODS The BMSCs were purified and amplified by methods of adherence and selection in vitro. One hundred and ninety rats were randomly divided into sham-operated group, untreated group, rhubarb aglycone group, BMSC transplantation group (abbreviated as transplantation group) and BMSCs combined with rhubarb aglycone group (abbreviated as combination group). Middle cerebral artery occlusion (MCAO) model was duplicated with nylon thread. Rats of transplantation and combination group were transplanted with BMSCs via carotid artery after 24-hour reperfusion. Rhubarb aglycone was used by intragastric administration in the rhubarb aglycone group and the combination group. The brain samples were taken at 7, 14 and 28 days after transplantation. Brain micrangium pathological changes were observed by light microscope, and immunohistochemical method was used to determine the expressions of immunoglobulin G (IgG), type IV collagen (Col IV), matrix metalloproteinase-9 (MMP-9), and tissue inhibitor of metalloproteinase-1 (TIMP-1). RESULTS Comparison with the normal control group revealed that brain micrangium in rats in the untreated group was obviously mutilated and damaged, the expression of IgG and MMP-9 increased, and showed a progressively enhanced tendency following the prolongation of reperfusion, while the expressions of Col IV and TIMP-1 were decreased, and TIMP-1 showed a attenuated tendency following the prolongation of reperfusion. Comparing with the untreated group, the improvements of brain micrangium structure in the rhubarb aglycone group (7 days after transplantation), the transplantation group (14 and 28 days after transplantation) and the combination group were significant; expression of IgG and activity of MMP-9 were decreased, while expressions of Col IV and TIMP-1 were increased in the rhubarb aglycone group and the combination group at each time point. The brain micrangium was integral and the expression of Col IV was enhanced in combination group (7 days after transplantation) as compared with those in transplantation group. MMP-9 activity in combination group (14 days after transplantation) was lower than that in the rhubarb aglycone group (14 days after transplantation), while expression of TIMP-1 in combination group also increased significantly as compared with that in transplantation group (28 days after transplantation). CONCLUSION Rhubarb aglycone can decrease the degradation of basal lamina Col IV and the permeability of brain micrangium in cerebral ischemic rats with BMSC transplantation, and its mechanisms may be related to regulating the balance of MMP-9, especially by increasing the expression of TIMP-1. |
| Databáze: | OpenAIRE |
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