Feasibility of the direct coupling of solid-phase extraction-pipette tips with a programmed-temperature vaporiser for gas chromatographic analysis of drugs in plasma
| Autor: | W. M. A. Van Egmond, G.J. de Jong, M.W.J van Hout, R.A. de Zeeuw, J.P. Franke |
|---|---|
| Přispěvatelé: | Pharmaceutical Analysis, Analytical Biochemistry |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Chromatography
Gas Clinical Biochemistry LEVEL Thermal desorption Analytical chemistry Biochemistry Sensitivity and Specificity Mass Spectrometry Analytical Chemistry PESTICIDES LARGE-VOLUME INJECTION Desorption WATER Solid phase extraction TANDEM MASS-SPECTROMETRY programmed-temperature vaporiser diazepam Detection limit Chromatography Chemistry Extraction (chemistry) Pipette Reproducibility of Results Cell Biology General Medicine THERMAL-DESORPTION solid-phase extraction-pipette tips coupling lidocaine Feasibility Studies Direct coupling MICROCONTAMINANTS Gas chromatography AQUEOUS SAMPLES |
| Zdroj: | Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 766(1), 37-45. ELSEVIER SCIENCE BV |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/s0378-4347(01)00431-5 |
| Popis: | Solid-phase extraction-pipette tips (SPE-PTs) were used for micro solid-phase extraction of lidocaine and diazepam from plasma. Off-line extraction was followed by on-line desorption. On-line desorption was carried out by direct coupling of the SPE-PTs with the liner of the programmed-temperature vaporiser. This coupling only required shortening of the liner by maximally 16 mm, cutting the SPE-PT, and equipping the remaining part with two O-rings. Due to the heating of the injector the SPE-PTs were heated as well, which resulted in a significant amount of impurities. Pre-heating and pre-washing was performed prior to the extraction to reduce the impurity level. The internal coupling device was applied successfully for the analysis of plasma samples with gas chromatography (GC) and mass-selective detection. Detection limits of 0.75 ng/ml and 2.5 ng/ml were obtained for lidocaine and diazepam, respectively, using 200 mul plasma. Recoveries for both compounds were about 80%. Although it is possible, the internal coupling device was not developed to be used as such. The main goal of this coupling was to show the feasibility of the integration of SPE-PTs with GC and to realize an important step to new automated SPE-GC systems. (C) 2002 Elsevier Science B.V. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect