Neuroparsin transcripts as molecular markers in the process of desert locust (Schistocerca gregaria) phase transition
| Autor: | Filip Sas, Ilse Claeys, Bert Breugelmans, Jozef Vanden Broeck, Gert Simonet, Arnold De Loof, Sofie Van Soest |
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| Rok vydání: | 2006 |
| Předmět: |
Genetic Markers
Male Fat body DNA Complementary Time Factors animal structures Peptide Hormones media_common.quotation_subject Molecular Sequence Data Biophysics Grasshoppers Insect Biochemistry Polyphenism Animals Tissue Distribution RNA Messenger Protein Precursors Desert locust Molecular Biology DNA Primers media_common Cdna cloning Base Sequence biology Reverse Transcriptase Polymerase Chain Reaction Ecology Metamorphosis Biological Cell Biology Migratory locust biology.organism_classification Evolutionary biology Insect Hormones Female Schistocerca Locust |
| Zdroj: | Biochemical and Biophysical Research Communications. 341:599-606 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2006.01.011 |
| Popis: | Desert locust swarms occasionally cause severe ecological and economic damage, particularly in countries of northwest Africa. However, the physiological mechanisms underlying locust phase transition, the switch of the solitarious to the gregarious phase, remain elusive. Therefore, identification of molecular changes linked to this phenomenon represents a primary requirement to start unraveling this enigma. The present paper provides novel information on phase-related molecular markers for locust phase transition. We present a detailed quantitative real-time RT-PCR analysis of two distinct neuroparsin precursor transcripts (Scg-NPP3 and Scg-NPP4) in the brain and in abdominal tissues of gregarious and solitarious desert locusts (Schistocerca gregaria). Our data reveal different temporal changes of these transcripts in the fat body during the adult stage of both phases. We, hereby, present novel scientific evidence for a phase-dependent regulation of these particular peptide hormone encoding transcripts and assign them as possible molecular markers in the process of locust phase transition. |
| Databáze: | OpenAIRE |
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