DNA Repair in Congenic Mice: Possible Influence of a Chromosome 4 Genetic Region on the Rate of Benzo[a]pyrene-Induced DNA Adduct Removal
| Autor: | Jeanne Y. Wei, Michael E.T.I. Boerrigter, Yi Yin, Jan Vijg |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Aging DNA Repair DNA repair 7 8-Dihydro-7 8-dihydroxybenzo(a)pyrene 9 10-oxide Congenic Mice Inbred Strains Biology Chromosomes DNA Adducts Mice chemistry.chemical_compound Life Expectancy DNA adduct Benzo(a)pyrene Animals Benzopyrenes Gene Mice Inbred BALB C Nucleotides Deoxyguanosine DNA Molecular biology Mice Inbred C57BL Chromosome 4 Genes Liver chemistry Chromosomal region DNA Damage |
| Zdroj: | Journal of Gerontology. 48:B11-B16 |
| ISSN: | 0022-1422 |
| DOI: | 10.1093/geronj/48.1.b11 |
| Popis: | An attempt was made to assign mouse lifespan-associated interstrain differences in DNA repair to a specific chromosomal region using a set of congenic mice. The sensitive 32P-postlabeling assay was employed to measure the removal of benzo[a]pyrene-induced DNA adducts in liver DNA of three different chromosome 4 congenic mouse strains: B6.C-H-15c, B6.C-H-16c, and B6.C-H-26c and the two parental strains, C57B1/6 and BALB/c. The removal of the one main adduct detected, trans-(7R)-N2-[10-(7 beta,8 alpha,9 alpha-trihydroxy)-7,8,9,10- tetrahydrobenzo(a)-pyrene]-yl-deoxyguanosine (BPDE-N2-dG), in liver DNA of C57Bl/6 and BALB/c mice between one and three days after treatment, was approximately 86% and 57%, respectively. The percentage removal of BPDE-N2-dG in two of the three congenic mouse strains, B6.C-H-16c and B6.C-H-26c, resembled that found in BALB/c, whereas the third strain, B6.C-H-15c, removed about the same amount as C57B1/6, i.e., approximately 88% of BPDE-N2-dG between one and three days after treatment. The usefulness of congenic mouse strains for identifying genes putatively involved in aging and/or disease susceptibility is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|