Generation of a PDX1-EGFP reporter human induced pluripotent stem cell line, KSCBi005-A-3, using the CRISPR/Cas9 system
| Autor: | Mi-Hyun Park, Ji-Won Kim, Ara Kwon, Hyeyeon Park, Soo Kyung Koo, Hye Young Choi, Min Jung Kim, Young-Sun Lee, Yong-Ou Kim, Sungwook Kwak |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Green Fluorescent Proteins Cell Culture Techniques Biology Cell Line 03 medical and health sciences 0302 clinical medicine Genes Reporter CRISPR Humans Induced pluripotent stem cell lcsh:QH301-705.5 Transcription factor Homeodomain Proteins Base Sequence Cas9 Cell Biology General Medicine Cell biology 030104 developmental biology lcsh:Biology (General) Cell culture Trans-Activators PDX1 Stem cell CRISPR-Cas Systems Homologous recombination 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 41, Iss, Pp-(2019) |
| ISSN: | 1876-7753 |
| Popis: | PDX1 plays a crucial role in the development and maintenance of β-cells and directly regulates pancreatic β-cell-specific transcription factors by binding to the insulin gene. Here, we introduced an EGFP reporter into the C-terminus of PDX1 in KSCBi005-A human induced pluripotent stem cells through homologous recombination using CRISPR/Cas9 nuclease. The cells had a normal karyotype, expressed several pluripotency markers, and maintained their differentiation potential. KSCBi005-A-3 cells can be used to monitor PDX1 expression in live cells during β-cell differentiation; the cell line has been registered at the National Stem Cell Bank, Korea National Institute of Health. |
| Databáze: | OpenAIRE |
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