First outbreak of an infection with infectious spleen and kidney necrosis virus (ISKNV) in ornamental fish in Germany
| Autor: | Jan Wolter, Verena Jung-Schroers, Dieter Steinhagen, Mikolaj Adamek, Helmut Wedekind, Peter Wohlsein |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Iridoviridae Necrosis Spleen Aquaculture Aquatic Science Colombia Megalocytivirus Virus 03 medical and health sciences Fish Diseases Germany medicine Animals Ecology Evolution Behavior and Systematics biology Pterophyllum Commerce Fishes Outbreak biology.organism_classification Virology DNA Virus Infections 030104 developmental biology medicine.anatomical_structure medicine.symptom Kidney necrosis |
| Zdroj: | Diseases of aquatic organisms. 119(3) |
| ISSN: | 0177-5103 |
| Popis: | In 2014, infectious spleen and kidney necrosis virus (ISKNV), a member of the genus Megalocytivirus, was detected for the first time in ornamental fish in Germany. Since 2013, angelfish Pterophyllum spp. originating from Colombia have experienced significant epizootics in a number of German retailers' facilities. The diseased fish showed symptoms such as increased ventilation, swollen gills, and ulcerations of the skin. In 2014, diseased angelfish P. altum and platys Xiphophorus maculatus maintained in the same recirculating system were examined. Histopathological lesions included hypertrophic cells, single-cell necrosis, and an inflammatory infiltration of granulocytes, lymphocytes, and macrophages in liver, spleen, and kidney. Transmission electron microscopy revealed the presence of numerous polygonal viral particles (150 nm in diameter) within the cytoplasm of enlarged cells. A PCR assay for the detection of megalocytiviruses amplified 777 bp of major capsid protein gene that was 100% identical to ISKNV. This is the first report of an ISKNV outbreak in Germany that most probably was introduced by infected angelfish from Colombia. To our knowledge, this is the first report of ISKNV detected in fish imported from South America. Given the lethal nature of megalocytiviruses, proper biosecurity would seem prudent in countries like Germany where these emerging pathogens are not established. |
| Databáze: | OpenAIRE |
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