Adenosine A2A receptor contributes to ischemic brain damage in newborn piglet
| Autor: | Raymond C. Koehler, Zeng Jin Yang, Kerry D. Heitmiller, Abby C. Larson, Erin L. Carter, Gina Hong, Lee J. Martin, Bing Wang, Herman Kwansa, Jessica L. Jamrogowicz |
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| Rok vydání: | 2013 |
| Předmět: |
Agonist
Male medicine.medical_specialty Adenosine Adenosine A2 Receptor Agonists Receptor Adenosine A2A medicine.drug_class Sus scrofa Adenosine A2A receptor Biology SCH-58261 chemistry.chemical_compound Internal medicine Phenethylamines medicine Laser-Doppler Flowmetry Animals Receptor CGS-21680 Neurons Triazoles Receptor antagonist Immunohistochemistry Corpus Striatum Adenosine A2 Receptor Antagonists Disease Models Animal Oxidative Stress Endocrinology Pyrimidines Neurology chemistry nervous system Animals Newborn Cerebrovascular Circulation Hypoxia-Ischemia Brain NMDA receptor Original Article Neurology (clinical) Cardiology and Cardiovascular Medicine Blood Flow Velocity medicine.drug |
| Zdroj: | Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 33(10) |
| ISSN: | 1559-7016 |
| Popis: | Pharmacologic inactivation or genetic deletion of adenosine A2A receptors protects ischemic neurons in adult animals, but studies in neonatal hypoxia-ischemia (H-I) are inconclusive. The present study in neonatal piglets examined the hypothesis that A2A receptor signaling after reoxygenation from global H-I contributes to injury in highly vulnerable striatal neurons where A2A receptors are enriched. A2A receptor immunoreactivity was detected in striatopallidal neurons. In nonischemic piglets, direct infusion of the selective A2A receptor agonist CGS 21680 through microdialysis probes into putamen increased phosphorylation of N-methyl-D-aspartic acid (NMDA) receptor NR1 subunit and Na+, K+-ATPase selectively at protein kinase A (PKA)-sensitive sites. In ischemic piglets, posttreatment with SCH 58261, a selective A2A receptor antagonist, improved early neurologic recovery and preferentially protected striatopallidal neurons. SCH 58261 selectively inhibited the ischemia-induced phosphorylation of NR1, Na+, K+-ATPase, and cAMP-regulated phosphoprotein 32 KDa (DARPP32) at PKA-sensitive sites at 3 hours of recovery and improved Na+, K+-ATPase activity. SCH 58261 also suppressed ischemia-induced protein nitration and oxidation. Thus, A2A receptor activation during reoxygenation contributes to the loss of a subpopulation of neonatal putamen neurons after H-I. Its toxic signaling may be related to DARPP32-dependent phosphorylation of PKA-sensitive sites on NR1 and Na+, K+-ATPase, thereby augmenting excitotoxicity-induced oxidative stress after reoxygenation. |
| Databáze: | OpenAIRE |
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