Significant changes in circular RNA in the mouse cerebral cortex around an injury site after traumatic brain injury
| Autor: | Chongjie Cheng, Yue Wu, Junqing Yang, Xiaocui Tian, Zhijian Huang, Xiaochuan Sun, Zongduo Guo, Hong Wang, Jianjun Zhong, Li Jiang, Rami Darwazeh, Zhihao Chen |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Traumatic brain injury Receptors Interleukin-8B 03 medical and health sciences Mice 0302 clinical medicine Injury Site Developmental Neuroscience Circular RNA Cortex (anatomy) microRNA Brain Injuries Traumatic medicine Animals Cells Cultured Cerebral Cortex Inflammation business.industry Penumbra RNA RNA Circular medicine.disease Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Neurology Cerebral cortex business 030217 neurology & neurosurgery Psychomotor Performance |
| Zdroj: | Experimental neurology. 313 |
| ISSN: | 1090-2430 |
| Popis: | Background and objective Circular RNA (circRNA) is an important type of non-coding RNA that has not been widely researched in traumatic brain injury (TBI). The present study aimd to detect the altered circRNA expression around an injury site in the mouse cerebral cortex after TBI and explore its potential functions. Method C57BL/6 mice were used to construct a controlled cortical impact (CCI) model to simulate TBI. At 24 h post-TBI, the cortex around the injury site was collected, and the total RNA was extracted to perform RNA sequencing (RNA-seq). The differentially expressed circRNAs were determined according to the following criteria: |log2(fold change)| > 1, P Results A total of 8036 altered circRNAs were discovered, and among them, 16 were significantly changed (5 up-regulated and 11 down-regulated). The circRNA chr8_87,859,283–87,904,548 significantly increased by approximately 4 times in the cerebral cortex around the injury site after TBI and promoted neuro-inflammation through increasing the CXCR2 protein by sponging mmu-let-7a-5p. As a result, the increased circRNA chr8_87,859,283–87,904,548 blocked the restoration of neurological function after TBI. Conclusion Many circRNAs are significantly up-regulated or down-regulated in the traumatic cerebral penumbra cortex after TBI. Among them, the circRNA chr8_87,859,283–87,904,548 potentially plays a pro-inflammatory role, which may have a deleterious effect on neurological restoration after TBI. . |
| Databáze: | OpenAIRE |
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