Quinolobactin, a new siderophore of Pseudomonas fluorescens ATCC 17400, the production of which is repressed by the cognate pyoverdine
| Autor: | Ulrich Wolff, Nico Koedam, Dimitris Mossialos, Herbert Budzikiewicz, Jean-Marie Meyer, Christine Baysse, Pierre Cornelis, Vanamala Anjaiah |
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| Rok vydání: | 2000 |
| Předmět: |
Siderophore
Iron Siderophores Pseudomonas fluorescens Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Ferrichrome chemistry.chemical_classification Pyoverdine Ecology biology Pigments Biological biology.organism_classification Physiology and Biotechnology Amino acid Culture Media Mutagenesis Insertional chemistry Biochemistry Pseudomonadales DNA Transposable Elements Quinolines Electrophoresis Polyacrylamide Gel Oligopeptides Salicylic acid Food Science Biotechnology Pseudomonadaceae Bacterial Outer Membrane Proteins |
| Zdroj: | Applied and environmental microbiology. 66(2) |
| ISSN: | 0099-2240 |
| Popis: | Several species of rRNA group I pseudomonads (the genus Pseudomonas sensu stricto) are characterized, under iron-limiting conditions, by the production of fluorescent, yellow-green, specific iron(III) chelators (siderophores) that are called pyoverdines or pseudobactins (1, 2, 12). Each of these siderophores, which are needed for high-affinity transport of iron(III) to the cell (16), is composed of a dihydroxyquinoline chromophore and a variable peptide chain comprising 6 to 12 amino acids, depending on the producing strain (2). In addition to these high-affinity iron chelators, fluorescent pseudomonads are known to produce other lower-affinity siderophores, such as pyochelin, a derivative of salicylic acid (7), and salicylic acid itself (18, 25). Fluorescent pseudomonads are also characterized by their capacity to take up a variety of structurally unrelated siderophores, including pyoverdines (pseudobactins) produced by other strains (3, 14). When these heterologous siderophores are added to a culture, they induce the production of corresponding siderophore receptors in the outer membrane via a signal cascade relayed by the receptor itself (10, 14). Pseudomonas fluorescens ATCC 17400 has been studied in our laboratory as a bacterium that is able to utilize different siderophores, including ferrichrome, deferrioxamine, pseudobactin BN7, and B10 (unpublished results). This bacterium also exhibits iron-repressed in vitro antagonism against the phytopathogen Pythium debaryanum (6). It has been demonstrated that iron-deprived P. fluorescens ATCC 17400 cells produce not only a specific pyoverdine whose structure is known (8) but also 8-hydroxy-4-methoxy-monothioquinaldic acid, which is readily hydrolyzed in the culture medium to 8-hydroxy-4-methoxy-quinaldic acid (20). A pyoverdine-negative P. fluorescens Tn5 mutant of ATCC 17400 has been isolated in our laboratory (6). In this study, we demonstrated that this mutant produces 8-hydroxy-4-methoxy-quinaldic acid (renamed quinolobactin), which acts as a siderophore and induces a new iron-repressed outer membrane protein, and that the ferrisiderophore uptake system is preferentially induced in the absence of P. fluorescens wild-type pyoverdine. |
| Databáze: | OpenAIRE |
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