FOXO regulates cell fate specification of Drosophila ventral olfactory projection neurons
Autor: | Hung-Hsiang Yu, Jia-Yi Wei, Pei-Chi Chung, Sao-Yu Chu |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Neurogenesis Regulator Constitutively active Biology Cell fate determination Animals Genetically Modified 03 medical and health sciences Cellular and Molecular Neuroscience Functional brain 0302 clinical medicine Genetics Biological neural network Animals Drosophila Proteins Cell Lineage Gene Neurons fungi MARCM Cell Differentiation Forkhead Transcription Factors Olfactory Pathways Cell biology 030104 developmental biology 030217 neurology & neurosurgery Function (biology) |
Zdroj: | Journal of neurogenetics. 33(1) |
ISSN: | 1563-5260 |
Popis: | Diverse types of neurons must be specified in the developing brain to form the functional neural circuits that are necessary for the execution of daily tasks. Here, we describe the participation of Forkhead box class O (FOXO) in cell fate specification of a small subset of Drosophila ventral olfactory projection neurons (vPNs). Using the two-color labeling system, twin-spot MARCM, we determined the temporal birth order of each vPN type, and this characterization served as a foundation to investigate regulators of cell fate specification. Flies deficient for chinmo, a known temporal cell fate regulator, exhibited a partial loss of vPNs, suggesting that the gene plays a complex role in specifying vPN cell fate and is not the only regulator of this process. Interestingly, loss of foxo function resulted in the precocious appearance of late-born vPNs in place of early-born vPNs, whereas overexpression of constitutively active FOXO caused late-born vPNs to take on a morphology reminiscent of earlier born vPNs. Taken together, these data suggest that FOXO temporally regulates vPN cell fate specification. The comprehensive identification of molecules that regulate neuronal fate specification promises to provide a better understanding of the mechanisms governing the formation of functional brain tissue. |
Databáze: | OpenAIRE |
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