Degradation of immunoglobulin G by periodontal bacteria
| Autor: | H.J. Jansen, J. H. C. Göertz, J. A. J. M. Bakkeren, C. W. A. Kieboom, P.J.M. Camp, J. S. Hoeven |
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| Rok vydání: | 1994 |
| Předmět: |
Periodontium
Microbiology (medical) Immunology Oligosaccharides Enzyme-Linked Immunosorbent Assay Immunoelectrophoresis Streptococcus intermedius Microbiology Immunoglobulin G Antigen-Antibody Reactions Bacteria Anaerobic stomatognathic system medicine Actinomyces Bacteroides Humans Porphyromonas Symbiosis General Dentistry Porphyromonas gingivalis Ecosystem Fusobacterium nucleatum biology medicine.diagnostic_test Eubacterium Capnocytophaga ochracea Prevotella oralis Prevotella intermedia Streptococcus biology.organism_classification Antibodies Bacterial Culture Media Lactobacillus stomatognathic diseases Superinfection biology.protein Electrophoresis Polyacrylamide Gel Peptides Capnocytophaga |
| Zdroj: | Oral Microbiology and Immunology. 9:345-351 |
| ISSN: | 1399-302X 0902-0055 |
| DOI: | 10.1111/j.1399-302x.1994.tb00284.x |
| Popis: | Several subgingival microorganisms were tested for their ability to utilize human immunoglobulin G (IgG) as a substrate for growth. This was done using a protein-free chemically defined medium, supplemented with IgG. Stimulation of growth was observed for Capnocytophaga ochracea, Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella oralis, Lactobacillus catenaforme and Streptococcus intermedius. Immunoelectrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a protein assay demonstrated that P. intermedia and P. endodontalis completely degraded the protein chains of IgG. Partial breakdown of IgG was observed for P. asaccharolytica and C. ochracea, whereas P. oralis cleaved the IgG heavy chain, yielding Fc and Fab fragments. All these bacteria utilized IgG as a substrate for growth. Binding studies using an enzyme-linked immunosorbent assay, revealed complete loss of in vitro antigen-antibody binding capacity after incubation of specific IgG with P. endodontalis and partial loss of binding with P. intermedia, P. gingivalis, C. ochracea or Fusobacterium nucleatum. Degradation or inactivation of IgG by oral bacteria is thought to be important in the causation of polymicrobial infections. |
| Databáze: | OpenAIRE |
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