Protective effects of crocin against endogenous Aβ-induced neurotoxicity in N2a/APP695swe cells
| Autor: | Jinwen Li, Jikun Du, Yuanhua Li, Li Li, Daibo Song, Baohong Li, Qunfa Huang, Jierong Liu |
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| Rok vydání: | 2021 |
| Předmět: |
Pharmacology
Amyloid beta-Peptides Neurotoxicity Endogeny Apoptosis medicine.disease Neuroprotection Carotenoids 030227 psychiatry Crocin Pathogenesis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Neuroprotective Agents chemistry Annexin Alzheimer Disease medicine Humans Phosphorylation Cytotoxicity 030217 neurology & neurosurgery |
| Zdroj: | Psychopharmacology. 238(10) |
| ISSN: | 1432-2072 |
| Popis: | Alzheimer’s disease (AD) is a most common neurodegenerative disorder worldwide. Because of its complex pathogenesis, the prevention and therapies of AD still are a severe challenge. Evidence suggested that crocin, the major component of saffron, exhibited neuroprotective effects in AD. As such, in this study, N2a/APP695swe cells were enrolled to investigate the effects of crocin on endogenous Aβ-induced neurotoxicity. Crocin (100 and 200 μM) could ameliorate cytotoxicity according to CCK-8 assay and reduce apoptosis in line with Hoechst 33,342 staining and Annexin V-FITC/PI double staining in N2a/APP695swe cells. Reduced ROS generation and elevated MMP were found in N2a/APP695swe cells treated with crocin (100 and 200 μM). Additionally, crocin at concentrations of 100 and 200 μM inhibited the release of cytochrome and attenuated caspases-3 activity in N2a/APP695swe cells. Furthermore, succinylation, crotonylation, 2-hydroxyisobutyrylation, malonylation, and phosphorylation were significantly reduced, while a slight increase of acetylation was found in 100-μM crocin treated N2a/APP695swe cells. Taken together, crocin may be a promising natural product candidate for the effective cure of AD. |
| Databáze: | OpenAIRE |
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