Chitosan Stabilized Silver Nanoparticles for the Electrochemical Detection of Lipopolysaccharide: A Facile Biosensing Approach for Gram-Negative Bacteria
| Autor: | Christopher J. Ehrhardt, Vamsi K. Yadavalli, Muhammad R. Shah, Muhammad Imran, Massimo F. Bertino |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
silver nanoparticles
Gram-negative bacteria lcsh:Mechanical engineering and machinery 02 engineering and technology medicine.disease_cause biosensor 01 natural sciences Silver nanoparticle Article Chitosan chemistry.chemical_compound medicine Escherichia coli lcsh:TJ1-1570 Electrical and Electronic Engineering Chromatography biology Mechanical Engineering 010401 analytical chemistry lipopolysaccharide 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Membrane chemistry Control and Systems Engineering chitosan 0210 nano-technology Bacterial outer membrane Biosensor Bacteria |
| Zdroj: | Micromachines, Vol 11, Iss 413, p 413 (2020) Micromachines Volume 11 Issue 4 |
| Popis: | Negatively charged lipopolysaccharide (LPS), a major endotoxin and component of the outer membrane of several Gram-negative bacteria, provides a useful biomarker for the indirect detection of these pathogens. For instance, Escherichia coli (E. coli) is a pathogenic bacterium that causes infections in almost all age groups, and has been implicated in food and water contamination. Current diagnostic and detection methods tend to be labor-intensive or expensive, necessitating the need for an easy, sensitive, rapid, and low-cost method. We report on the synthesis and use of positively charged chitosan stabilized silver nanoparticles (Chi-AgNPs) as a sensitive electrochemical nanobiosensor for the detection of LPS. Chi-AgNPs were synthesized through a facile, single step protocol, and characterized for size, charge, and morphology. Glassy carbon electrodes modified with Chi-AgNPs resulted in an enhancement of signal in the presence of both LPS and E. coli. Detection was accomplished over a large concentration range (several orders of magnitude) of 0.001&ndash 100 ng/mL and 10&ndash 107 CFU/mL. The biosensors can reliably detect LPS and E. coli at very low concentrations. Chi-AgNPs have potential as low cost, sensitive nanobiosensors for Gram-negative bacteria due to strong electrostatic interaction with LPS present in their outer membranes. |
| Databáze: | OpenAIRE |
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