Temporal complexity within a translational control element in thenanosmRNA
| Autor: | Kevin M. Forrest, Elizabeth R. Gavis, Roshan A. Jain, Ira E. Clark |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Untranslated region
Cytoplasm Time Factors Transcription Genetic Green Fluorescent Proteins Immunoblotting Molecular Sequence Data Repressor Biology Animals Drosophila Proteins RNA Messenger Transgenes Luciferases 3' Untranslated Regions Molecular Biology Psychological repression Germ plasm Genetics Messenger RNA Binding Sites Base Sequence Models Genetic technology industry and agriculture Gene Expression Regulation Developmental RNA-Binding Proteins Translation (biology) Blotting Northern Embryonic stem cell Protein Structure Tertiary Cell biology Drosophila melanogaster Microscopy Fluorescence Protein Biosynthesis Mutation RNA Smaug Plasmids Protein Binding Developmental Biology |
| Zdroj: | Development. 131:5849-5857 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | Translational control of gene expression plays a fundamental role in the early development of many organisms. In Drosophila, selective translation of nanos mRNA localized to the germ plasm at the posterior of the embryo, together with translational repression of nanos in the bulk cytoplasm, is essential for development of the anteroposterior body pattern. We show that both components to spatial control of nanos translation initiate during oogenesis and that translational repression is initially independent of Smaug, an embryonic repressor of nanos. Repression during oogenesis and embryogenesis are mediated by distinct stem loops within the nanos 3′ untranslated region;the Smaug-binding stem-loop acts strictly in the embryo, whereas a second stem-loop functions in the oocyte. Thus, independent regulatory modules with temporally distinct activities contribute to spatial regulation of nanos translation. We propose that nanos evolved to exploit two different stage-specific translational regulatory mechanisms. |
| Databáze: | OpenAIRE |
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