High Performance Gene Delivery Polymeric Vector: Nano-Structured Cationic Star Polymers (Star Vectors)
| Autor: | Michiko Hayashi, Moto Ohira, Takeshi Masuda, Mariko Harada-Shiba, Makoto Nagaishi, Yasuhide Nakayama |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
chemistry.chemical_classification
Cell Survival Polymers Chemistry Genetic Vectors Gene Transfer Techniques Cationic polymerization Gene Expression Pharmaceutical Science Polymer Gene delivery Branching (polymer chemistry) Nanostructures chemistry.chemical_compound Polymerization Drug Design Acrylamide COS Cells Chlorocebus aethiops Polymer chemistry Nano Animals Technology Pharmaceutical Particle size Luciferases |
| Zdroj: | Current Drug Delivery. 2:53-57 |
| ISSN: | 1567-2018 |
| Popis: | Nano-structured hyperbranched cationic star polymers, called star vectors, were molecularly designed for a novel gene delivery non-viral vector. The linear and 3, 4 or 6 branched water-soluble cationic polymers, which had same molecular weight of ca. 18,000, were synthesized by iniferter (initiator-transfer agent-terminator)-based photo-living-radical polymerization of 3-(N,N-dimethylamino)propyl acrylamide, initiated from respective multi-dithiocarbamate-derivatized benzenes as an iniferter. All polymers produced polyion complexes 'polyplexes' by mixing with pDNA (pGL3-control plasmid), in which the particle size was ca. 250 nm in diameter [the charge ratio2/1 (vevtor/pDNA)] and ca. 150 nm (the charge ratio2.5/1), and the zeta-potential was ca. +10 mV (the charge ratio1/1). When COS-1 cells were incubated with the polyplexes 12 h after preparation under the charge ratio of 5/1, higher gene expression was obtained as an increase in branching, with a little cytotoxicity. The relative gene expression to the linear polymer was about 2, 5, and 10 times in 3-, 4-, and 6-branched polymers, respectively. The precise change in branching of polymers enabled the control of the gene transfer activity. |
| Databáze: | OpenAIRE |
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