Cellular localization of rapid RNA and protein synthesis induced in presensitized lymphocytes by a cationic protein from brain
| Autor: | Steven Kornguth, John Walberg Anderson, Hartwell G. Thompson, Judith L. Ladinsky |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
Lymphocyte
Freund's Adjuvant Guinea Pigs Protein metabolism Nerve Tissue Proteins Biology Fluorescence chemistry.chemical_compound Antigen Leucine Protein biosynthesis medicine Animals Lymphocytes Uridine Incubation Cellular localization Brain Chemistry Microscopy Messenger RNA Research Brain Proteins RNA Nucleosides Cell Biology Molecular biology Metabolism medicine.anatomical_structure Microscopy Fluorescence chemistry Biochemistry Protein Biosynthesis Autoradiography Lymph Nodes |
| Zdroj: | Experimental Cell Research. 37:650-661 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(65)90213-2 |
| Popis: | Results of previous biochemical experiments indicated that lymph node cells, presensitized to a purified cationic protein from guinea pig brain, were induced to undergo rapid protein synthesis when incubated in the presence of the basic protein. An incubation period of 1 hr was required before the increase in protein synthesis could be observed. In the autoradiographic studies here reported, the nuclei of presensitized cells which appear to be medium lymphocytes, gave evidence of rapid RNA synthesis (possibly messenger RNA) during the initial hour of incubation with the cationic protein. During the second hour of incubation the high rate of protein synthesis was observed in both small and medium lymphocytes. With the aid of fluorescent antigen it was demonstrated that the medium sized lymphocytes contain a material that specifically binds the cationic protein. The role of the medium lymphocyte in the induction of hypersensitive reactions is discussed. |
| Databáze: | OpenAIRE |
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