Detection of Bovine Leukemia Virus RNA in Blood Samples of Naturally Infected Dairy Cattle
| Autor: | Natalia Gabriela Porta, Karina Trono, Irene Alvarez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
POL GENE
040301 veterinary sciences TAX GENE animal diseases viruses Blood Sampling viral expression Biology Asymptomatic Article 0403 veterinary science Pathogenesis 03 medical and health sciences Virus Leucemia Bovina pol gene Dairy Cattle medicine RNA Viruses bovine leukemia virus RNA detection tax gene Dairy cattle 030304 developmental biology Whole blood 0303 health sciences lcsh:Veterinary medicine General Veterinary Bovine leukemia virus Oligonucleotide Ciencias Veterinarias VIRAL EXPRESSION RNA virus diseases 04 agricultural and veterinary sciences BOVINE LEUKEMIA VIRUS biology.organism_classification Virology Muestreo Sanguíneo CIENCIAS AGRÍCOLAS Ganado de Leche RNA DETECTION Bovine Leukaemia Virus lcsh:SF600-1100 medicine.symptom purl.org/becyt/ford/4.3 [https] purl.org/becyt/ford/4 [https] Blood sampling |
| Zdroj: | Veterinary Sciences, Vol 6, Iss 3, p 66 (2019) Veterinary Sciences CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Veterinary Sciences; Volume 6; Issue 3; Pages: 66 Veterinary sciences 6 (3) : 66. (Septiembre 2019) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA |
| ISSN: | 2306-7381 |
| Popis: | The viral expression in vivo, in bovine leukemia virus (BLV)-infected cattle, is considered to be restricted to extremely low levels, and the mitosis of infected B lymphocytes is regarded as the main mode of virus persistence within the infected host. In this study, the presence of BLV RNA in whole blood from seven asymptomatic cows naturally infected with BLV during one year, including a complete milking cycle and two delivery time points, was investigated by nested-PCR using the oligonucleotides complementary to the tax and pol gene. BLV RNA was detected in four cows at different time points, especially in high blood proviral load cows and around delivery time. This study describes the detection of free BLV RNA in blood from BLV-infected asymptomatic cows. The results obtained suggest the occurrence of persistent low-level expression of the tax and pol genes that could be a result of viral reactivation, within the asymptomatic period. This finding may be important in the pathogenesis of BLV infection, associated with the delivery period. Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Gastronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Porta, Natalia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Gastronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Virología e Innovaciones Tecnológicas; Argentina |
| Databáze: | OpenAIRE |
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