Developing Potential Candidates of Preclinical Preeclampsia
| Autor: | David L. Lykins, Xuemei Zeng, James M. Roberts, Sandra A. Founds |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Proteomics
preeclampsia candidate gene candidate protein biomarker development prevention Candidate gene Proteome Biology Bioinformatics Article Catalysis Preeclampsia lcsh:Chemistry Inorganic Chemistry Pre-Eclampsia Pregnancy Tandem Mass Spectrometry medicine Humans Genetic Predisposition to Disease Multiplex Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Genetic Association Studies Spectroscopy Organic Chemistry Trophoblast General Medicine medicine.disease 3. Good health Computer Science Applications medicine.anatomical_structure Targeted mass spectrometry lcsh:Biology (General) lcsh:QD1-999 Case-Control Studies biology.protein Female Antibody Peptides Biomarkers Chromatography Liquid |
| Zdroj: | International Journal of Molecular Sciences; Volume 16; Issue 11; Pages: 27208-27227 International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 16, Iss 11, Pp 27208-27227 (2015) |
| ISSN: | 1422-0067 |
| Popis: | The potential for developing molecules of interest in preclinical preeclampsia from candidate genes that were discovered on gene expression microarray analysis has been challenged by limited access to additional first trimester trophoblast and decidual tissues. The question of whether these candidates encode secreted proteins that may be detected in maternal circulation early in pregnancy has been investigated using various proteomic methods. Pilot studies utilizing mass spectrometry based proteomic assays, along with enzyme linked immunosorbent assays (ELISAs), and Western immunoblotting in first trimester samples are reported. The novel targeted mass spectrometry methods led to robust multiple reaction monitoring assays. Despite detection of several candidates in early gestation, challenges persist. Future antibody-based studies may lead to a novel multiplex protein panel for screening or detection to prevent or mitigate preeclampsia. |
| Databáze: | OpenAIRE |
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