Nitric oxide and cyclooxygenase may participate in the analgesic and anti-inflammatory effect of the cucurbitacins fraction from Wilbrandia ebracteata
| Autor: | Artur J. de Brum-Fernandes, Ronaldo A. Ribeiro, José Carlos da Silva Rocha, Mirna Marques Bezerra, Rodrigo Rebelo Peters, Jarbas Mota Siqueira-Junior, Mareni Rocha Farias, Rosa Maria Ribeiro-do-Valle, Francisco Airton Castro Rocha, Patricia Baier Krepsky |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Hot Temperature medicine.drug_class Analgesic Drug Evaluation Preclinical Arthritis Inflammation Pharmacology Nitric Oxide General Biochemistry Genetics and Molecular Biology Anti-inflammatory Nitric oxide Mice chemistry.chemical_compound Cell Movement In vivo Chlorocebus aethiops Animals Medicine Rats Wistar General Pharmacology Toxicology and Pharmaceutics Hot plate test Pain Measurement Analgesics Behavior Animal Dose-Response Relationship Drug biology Plant Extracts business.industry Anti-Inflammatory Agents Non-Steroidal General Medicine medicine.disease Arthritis Experimental Hindlimb Rats Cucurbitaceae chemistry Prostaglandin-Endoperoxide Synthases COS Cells biology.protein Joints Cyclooxygenase medicine.symptom business |
| Zdroj: | Life Sciences. 73:2185-2197 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(03)00602-7 |
| Popis: | Wilbrandia ebracteata is a medicinal plant from South America used in folk medicine for the treatment of chronic rheumatic diseases. We have shown that the high performance liquid chromatography-characterized (HPLC) dichloromethane fraction isolated from Wilbrandia ebracteata (WEDC) inhibits the parameters observed in experimental models of inflammation in vivo and in vitro. In the present study, we extend our previous observations on the analgesic effects of WEDC by investigating its actions using the hot plate test and zymosan-induced writhing test in mice, as well as zymosan-induced arthritis in rats evaluating articular inflammatory pain, cell migration and determination of NO release into the joint exudate. The effect of WEDC on the activity of COX-1 and COX-2 in vitro and its ulcerogenic capacity in vivo were also investigated. The oral treatment of the animals with WEDC (1-10 mg/kg) produced a significant, dose-dependent reduction of articular incapacitation and abdominal contortions in the writhing test. The same effect was not observed in the hot plate and rota-rod tests. WEDC also reduced nitrite release into the zymosan-inflamed joints. In the evaluation of COX activity, we observed that WEDC was able to selectively inhibit COX-2 but not COX-1 activity in COS-7 cells. Moreover, WEDC treatment did not show gastrointestinal toxicity. Our data confirm the anti-nociceptive activities of the WEDC and indicate that this effect could be associated with inhibition of cyclooxygenase-2 (COX-2) and nitric oxide release. The effects could be attributed to cucurbitacins since several of these were isolated from the WEDC. |
| Databáze: | OpenAIRE |
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