Autocrine regulation of milk secretion by a protein in milk
| Autor: | Caroline V. P. Addey, Colin J. Wilde, L M Boddy, Malcolm Peaker |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Whey protein Molecular Sequence Data Mammary gland Lactose Biology Biochemistry chemistry.chemical_compound Mammary Glands Animal fluids and secretions Pregnancy Lactation medicine Animals Amino Acid Sequence Autocrine signalling Molecular Biology Peptide sequence chemistry.chemical_classification Goats Caseins food and beverages Epithelial Cells Cell Biology Milk Proteins Amino acid Molecular Weight Milk Whey Proteins medicine.anatomical_structure chemistry Female Rabbits Research Article Explant culture |
| Zdroj: | Biochemical Journal. 305:51-58 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3050051 |
| Popis: | Frequency or completeness of milk removal from the lactating mammary gland regulates the rate of milk secretion by a mechanism which is local, chemical and inhibitory in nature. Screening of goat's milk proteins in rabbit mammary explant cultures identified a single whey protein of M(r) 7600 able to inhibit synthesis of milk constituents. The active whey protein, which we term FIL (Feedback inhibitor of Lactation), also decreased milk secretion temporarily when introduced into a mammary gland of lactating goats. FIL was synthesized by primary cultures of goat mammary epithelial cells, and was secreted vectorially together with other milk proteins. N-terminal amino acid sequencing indicated that it is a hitherto unknown protein. The evidence indicates that local regulation of milk secretion by milk removal is through autocrine feedback inhibition by this milk protein. |
| Databáze: | OpenAIRE |
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