Factors enhancing diacylglycerol acyltransferase activity in microsomes from cell-suspension cultures of oilseed rape
| Autor: | Susan D. Byers, André Laroche, Kevin C. Smith, Randall J. Weselake |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Clinical chemistry
Magnesium Chloride Brassica Endogeny Biology Biochemistry Suspension culture Diglycerides Phosphatidate Magnesium Sulfate Adenosine Triphosphate Cytosol Microsomes Coenzyme A Diacylglycerol O-Acyltransferase Phosphorylation Cells Cultured Phospholipids chemistry.chemical_classification Organic Chemistry Cell Biology biology.organism_classification Enzyme chemistry Microsome Acyltransferases Lipidology |
| Zdroj: | Lipids. 34:1143-1149 |
| ISSN: | 1558-9307 0024-4201 |
| DOI: | 10.1007/s11745-999-0465-6 |
| Popis: | Several factors, including an unidentified endogenous component, were found to stimulate microsomal diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) from a microspore-derived cell-suspension culture of oilseed rape (Brassica napus L. cv. Jet Neuf). At a concentration of 25 mM, MgSO4 and MgCl2 stimulated microsomal DGAT 25- and 10-fold, respectively. ATP and CoA at concentrations of 2 and 1 mM stimulated the enzyme 2.4- and 12-fold, respectively, although the effects were lessened in the presence of higher Mg2+ concentrations. Although microsomal DGAT activity was increased only slightly by the addition of exogenous sn-1,2-diacylglycerol to the reaction mixture, it was increased substantially by the addition of exogenous phosphatidate. sn-Glycerol-3-phosphate and other phospholipids tested did not have this stimulatory effect. DGAT activity did not decrease when microsomes were incubated with ATP in the presence of the cytosolic fraction. This fraction, however, contained a small organic compound(s) that stimulated microsomal DGAT activity. |
| Databáze: | OpenAIRE |
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