Digital and analog gene circuits for biotechnology
| Autor: | Roquet Nathaniel B, Timothy K. Lu |
|---|---|
| Přispěvatelé: | Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology. Research Laboratory of Electronics, Massachusetts Institute of Technology. Synthetic Biology Center, Roquet, Nathaniel Bernard, Lu, Timothy K. |
| Rok vydání: | 2013 |
| Předmět: |
Scope (project management)
business.industry Gene regulatory network Context (language use) General Medicine Biology Applied Microbiology and Biotechnology Field (computer science) Article Biotechnology Metabolic engineering Synthetic biology Variable (computer science) Metabolic Engineering Escherichia coli Molecular Medicine Gene Regulatory Networks Synthetic Biology Bioprocess business |
| Zdroj: | PMC |
| ISSN: | 1860-7314 |
| Popis: | Biotechnology offers the promise of valuable chemical production via microbial processing of renewable and inexpensive substrates. Thus far, static metabolic engineering strategies have enabled this field to advance industrial applications. However, the industrial scaling of statically engineered microbes inevitably creates inefficiencies due to variable conditions present in large-scale microbial cultures. Synthetic gene circuits that dynamically sense and regulate different molecules can resolve this issue by enabling cells to continuously adapt to variable conditions. These circuits also have the potential to enable next-generation production programs capable of autonomous transitioning between steps in a bioprocess. Here, we review the design and application of two main classes of dynamic gene circuits, digital and analog, for biotechnology. Within the context of these classes, we also discuss the potential benefits of digital-analog interconversion, memory, and multi-signal integration. Though synthetic gene circuits have largely been applied for cellular computation to date, we envision that utilizing them in biotechnology will enhance the efficiency and scope of biochemical production with living cells. National Institutes of Health (U.S.) (1DP2OD008435) National Institutes of Health (U.S.) (1P50GM098792) National Science Foundation (U.S.) (1124247) United States. Defense Advanced Research Projects Agency Ellison Medical Foundation United States. Office of Naval Research (N00014-11-1-0725) United States. Office of Naval Research (N00014-11-1-0687) United States. Office of Naval Research (N00014-13-1-0424) United States. Army Research Office (W911NF-11-1-0281) Presidential Early Career Award for Scientists and Engineers Ford Foundation |
| Databáze: | OpenAIRE |
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