Antithrombin-Independent Effects of Heparins on Fibrin Clot Nanostructure
| Autor: | Christelle Yeromonahos, Raphaël Marlu, Benoît Polack, François Caton |
|---|---|
| Přispěvatelé: | Laboratoire de rhéologie (LR), Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique de Grenoble (INPG)-Université Joseph Fourier - Grenoble 1 (UJF), TheREx, Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), inconnu, Inconnu |
| Rok vydání: | 2012 |
| Předmět: |
MESH: Heparin
Nanostructure [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Nanotechnology 030204 cardiovascular system & hematology Fondaparinux Fibrinogen [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity Fibrin MESH: Thrombin 03 medical and health sciences 0302 clinical medicine Thrombin Fibrinolytic Agents medicine MESH: Fibrinolytic Agents Humans MESH: Thrombosis MESH: Fibrin Blood Coagulation ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences MESH: Humans MESH: Spectrophotometry biology Heparin Chemistry Antithrombin Thrombosis [CHIM.MATE]Chemical Sciences/Material chemistry [INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation 3. Good health [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry Spectrophotometry MESH: Blood Coagulation MESH: Fibrinogen Biophysics biology.protein [PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph] Cardiology and Cardiovascular Medicine Fibrinolytic agent medicine.drug |
| Zdroj: | Europe PubMed Central Arteriosclerosis, Thrombosis, and Vascular Biology Arteriosclerosis, Thrombosis, and Vascular Biology, American Heart Association, 2012, 32 (5), pp.1320-4. ⟨10.1161/ATVBAHA.112.245308⟩ Arteriosclerosis, Thrombosis, and Vascular Biology, American Heart Association, 2012, 32 (5), pp.1320-1324. ⟨10.1161/ATVBAHA.112.245308⟩ |
| ISSN: | 1524-4636 1079-5642 |
| Popis: | Objective— Because of the widespread clinical use of heparins, their effects on the enzymatic cascade are very well known. In contrast, little is known about the direct effect of heparins on the nanostructure of fibrin fibers, even though this nanostructure plays a major role in the mechanical strength and lysis of clots. This lack of reliable data can be correlated with the lack of a nonintrusive, quantitative method to determine this structure. We recently developed such a method that allows the simultaneous determination of the average fiber radius and the protein content using spectrometric data. In this study, we assessed the nanostructure of fibrin in a system composed of human thrombin and fibrinogen. Methods and Results— We provide quantitative evidence showing that both unfractionated heparin and low molecular weight heparin directly alter the nanostructure of fibrin fibers independent of their other actions on the coagulation cascade; as expected, the pentasaccharide fondaparinux has no effect. Conclusion— Our results show that in addition to the effect of heparin on the coagulation cascade, modifications of the fibrin nanostructure may also contribute to improved fibrinolysis. |
| Databáze: | OpenAIRE |
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