Small Acid-Soluble Proteins with Intrinsic Disorder Are Required for UV Resistance in Myxococcus xanthus Spores
Autor: | Daniel Fordice, John L. Dahl |
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Rok vydání: | 2011 |
Předmět: |
Myxococcus xanthus
Time Factors Ultraviolet Rays Bacillus subtilis Microbiology Microbial Cell Biology Cell wall chemistry.chemical_compound Bacterial Proteins Sporogenesis Molecular Biology Spores Bacterial biology fungi Gene Expression Regulation Bacterial biology.organism_classification Spore Cell biology chemistry Mutation bacteria Electrophoresis Polyacrylamide Gel Myxococcaceae Acids DNA Bacteria |
Zdroj: | Journal of Bacteriology. 193:3042-3048 |
ISSN: | 1098-5530 0021-9193 |
Popis: | Bacterial sporulation in Gram-positive bacteria results in s mall a cid- s oluble p roteins called SASPs that bind to DNA and prevent the damaging effects of UV radiation. Orthologs of Bacillus subtilis genes encoding SASPs can be found in many sporulating and nonsporulating bacteria, but they are noticeably absent from spore-forming, Gram-negative Myxococcus xanthus . This is despite the fact that M. xanthus can form UV-resistant spores. Here we report evidence that M. xanthus produces its own unique group of low-molecular-weight, acid-soluble proteins that facilitate UV resistance in spores. These M. xanthus -specific SASPs vary depending upon whether spore formation is induced by starvation inside cell aggregations of fruiting bodies or is induced artificially by glycerol induction. Molecular predictions indicate that M. xanthus SASPs may have some association with the cell walls of M. xanthus spores, which may signify a different mechanism of UV protection than that seen in Gram-positive spores. |
Databáze: | OpenAIRE |
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