Effect of consanguinity on Argentinean Angus beef DNA traceability
| Autor: | E. Beretta, A.S. Mello Cesar, Guillermo Giovambattista, Juan Pedro Lirón, N. Sorarrain, A. Rogberg-Muñoz, Alberto José Prando, Pilar Peral-García, Andrés Baldo, María Verónica Ripoli, Roberto José Antonio Vaca, P. Ramelli, Diego Manuel Posik, Enrique Jorge Pofcher, Pilar Drummond Sampaio Corrêa Mariani |
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| Rok vydání: | 2009 |
| Předmět: |
Genetic Markers
Veterinary medicine Otras Biotecnología Agropecuaria Meat Biotecnología Agropecuaria Argentina Consanguinity Biology Beef cattle TRACEABILITY Animals Inbreeding EXTENSIVE FARMING Animal Husbandry business.industry DNA TYPING DNA Animal husbandry Breed Biotechnology CONSANGUINITY Genetic marker CIENCIAS AGRÍCOLAS Microsatellite BOVINE Livestock Cattle business Food Science Microsatellite Repeats |
| Zdroj: | Meat science. 85(4) |
| ISSN: | 1873-4138 |
| Popis: | Since the 1990s several authors have envisaged the use of DNA to certify meat origin. Two major parameters must be assessed before a DNA based traceability protocol can be implemented in the food chain: (i) the information content of a DNA marker set in a specific livestock breed or group of breeds; (ii) the minimum number of DNA markers needed to obtain a statistically acceptable match probability. The objective of the present work was to establish the effect of different levels of inbreeding in the matching efficiency, and the minimum number of microsatellite markers needed, in a DNA based meat traceability program, starting from an 11-microsatellite marker panel. Samples were obtained from beef production farms in South America, where animals are typically bred under pasture-based extensive conditions. Three groups of animals with different consanguinity rates were sampled. Exclusion power (Q) was higher than 0.999998 and match probability lower than 3.01E-08, for the whole set of markers within each group. Both values were affected by consanguinity. To reach a two mismatch criteria exclusion power (Q2) of 99.99, six markers were needed in unrelated animals whereas seven markers were needed in related animals. To reach Q2=99.9999, 8 and 10 microsatellite markers, respectively, were needed. In general, one or two more microsatellite markers were needed to identify consanguineous animals. This study proved the DNA marker set used to be suitable for the identification of the meat from all slaughtered animals in Argentina, per week, month, and year. © 2010 Elsevier Ltd. Fil: Baldo, Andres. Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata; Argentina Fil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Prando, Alberto José. Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata; Argentina Fil: Mello Cesar, A.S.. Functional Genomics Laboratory; Italia Fil: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Sorarrain, Nicolas. Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata; Argentina Fil: Ramelli, P.. Functional Genomics Laboratory; Italia Fil: Posik, Diego Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina Fil: Pofcher, Enrique Jorge. Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata; Argentina Fil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Beretta, E.. Consorzio Qualità Della Carne Bovina c/o Coldiretti; Italia Fil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Vaca, R.. Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata; Argentina Fil: Mariani, P.. Functional Genomics Laboratory; Italia Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina |
| Databáze: | OpenAIRE |
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