Altrenogest during early pregnancy modulates uterine glandular epithelium and endometrial growth factor expression at the time implantation in pigs
| Autor: | Rafaella Fernandes Carnevale, Ricardo de Francisco Strefezzi, Priscila Viau, D. F. Leal, B. B. D. Muro, Denis Hideki Nakasone, Raquel Vasconcelos Guimarães de Castro, André P. Poor, Maitê Vidal Mendonça, Lidia Hildebrand Pulz, Simone Maria Massami Kitamura Martins, Cláudio Alvarenga de Oliveira, Fabiana Fernandes Bressan, M. S. Monteiro, Brendon Willian Bessi, André Furugen Cesar de Andrade, Glen W. Almond, Mariana Andrade Torres, Cristian Hernando Garcia Martinez, Gisele Mouro Ravagnani |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Altrenogest
medicine.medical_treatment Early pregnancy factor Biology progesterone Endometrium Andrology corpus luteum 03 medical and health sciences Glandular epithelium chemistry.chemical_compound 0302 clinical medicine medicine endometrium Pregnancy 030219 obstetrics & reproductive medicine General Veterinary Growth factor 0402 animal and dairy science 04 agricultural and veterinary sciences gilts medicine.disease 040201 dairy & animal science IGF-I medicine.anatomical_structure chemistry CORPO LÚTEO biology.protein Immunohistochemistry Animal Science and Zoology Original Article Corpus luteum |
| Zdroj: | Animal Reproduction, Volume: 18, Issue: 1, Article number: e20200431, Published: 28 MAY 2021 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Animal Reproduction Muro, B B D, Leal, D F, Carnevale, R F, Torres, M A, Mendonça, M V, Nakasone, D H, Martinez, C H G, Ravagnani, G M, Monteiro, M S, Poor, A P, Martins, S M M K, Viau, P, de Oliveira, C A, de Castro, R V G, Bessi, B W, Bressan, F F, Pulz, L H, Strefezzi, R F, Almond, G W & de Andrade, A F C 2021, ' Altrenogest during early pregnancy modulates uterine glandular epithelium and endometrial growth factor expression at the time implantation in pigs ', Animal reproduction, vol. 18, no. 1, e20200431 . https://doi.org/10.1590/1984-3143-AR2020-0431 |
| Popis: | This study evaluated the effects of supplying altrenogest from day 6-12 of pregnancy on the endometrial glandular epithelium, corpora lutea (CL) morphology, and endometrial and CL gene expression. A total of 12 crossbred females (Landrace × Large White) were used. The females were assigned to 4 treatments according to a random design with a 2 × 2 factorial arrangement, with two categories (sow or gilt) and two treatments (non-treated and treated with altrenogest). On day 6 of pregnancy, animals were allocated to one of the following groups: non-treated (NT, n = 6; 3 sows and 3 gilts), and (T, n = 6; 3 sows and 3 gilts) treated daily with 20 mg of altrenogest, from day 6-12 of pregnancy. All animals were euthanized on day 13 of pregnancy. All CLs were individually weighed, and their volume were determined. The endometrial glandular density (GD), mean glandular area (MGA), and vascular density (VD) were determined by histomorphometric and immunohistochemical analyses. Endometrium samples were collected and analyzed by qRT-PCR to evaluate the abundance of transcripts for VEGF and IGF-I. Females in the T group had higher MGA (P < 0.05) compared to the NT group. There was no effect of treatment on GD or VD for both experimental groups. Sows in the T group had augmented expression of IGF-I (P < 0.05). Progestagen had no detrimental effect on CL morphology. In conclusion, altrenogest improves the uterine environment during the peri-implantation period in pigs without compromising corpora lutea development. |
| Databáze: | OpenAIRE |
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