ATP and repetitive electric stimulation increases leukemia inhibitory factor expression in astrocytes: A potential role for astrocytes in the action mechanism of electroconvulsive therapy
| Autor: | Soichiro Maruyama, Akitoyo Hishimoto, Akira Monji, Ichiro Sora, Ikuo Otsuka, Tetsufumi Kanazawa, Hiroshi Yoneda, Satoshi Okazaki, Shuken Boku, Hiroki Kikuyama, Yoshito Mizoguchi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Hippocampal formation
Fibroblast growth factor Leukemia Inhibitory Factor 03 medical and health sciences Mice 0302 clinical medicine Adenosine Triphosphate Prosencephalon medicine Animals RNA Messenger Receptor Electroconvulsive Therapy Gene knockdown Chemistry General Neuroscience Neurogenesis Wnt signaling pathway General Medicine biological factors Electric Stimulation 030227 psychiatry Cell biology Electrophysiological Phenomena Mice Inbred C57BL Psychiatry and Mental health medicine.anatomical_structure Neurology Animals Newborn Astrocytes embryonic structures Fibroblast Growth Factor 2 Neurology (clinical) Leukemia inhibitory factor 030217 neurology & neurosurgery Astrocyte Receptors Purinergic P2X2 |
| Zdroj: | Psychiatry and clinical neurosciencesReferences. 74(5) |
| ISSN: | 1440-1819 |
| Popis: | Aim Electroconvulsive therapy (ECT) is effective for psychiatric disorders. However, its action mechanism remains unclear. We previously reported that transcription factor 7 (TCF7) was increased in patients successfully treated with ECT. TCF7 regulates Wnt pathway, which regulates adult hippocampal neurogenesis. Adult hippocampal neurogenesis is involved in the pathophysiology of psychiatric disorders. Astrocytes play a role in adult hippocampal neurogenesis via neurogenic factors. Of astrocyte-derived neurogenic factors, leukemia inhibitory factor (LIF) and fibroblast growth factor 2 (FGF2) activate Wnt pathway. In addition, adenosine triphosphate (ATP), released from excited neurons, activates astrocytes. Therefore, we hypothesized that ECT might increase LIF and/or FGF2 in astrocytes. To test this, we investigated the effects of ATP and electric stimulation (ES) on LIF and FGF2 expressions in astrocytes. Methods Astrocytes were derived from neonatal mouse forebrain and administered ATP and ES. The mRNA expression was estimated with quantitative reverse-transcription polymerase chain reaction. Protein concentration was measured with ELISA. Results ATP increased LIF, but not FGF2, expression. Multiple ES, but not single, increased LIF expression. Knockdown of P2X2 receptor (P2X2R) attenuated ATP-induced increase of LIF mRNA expression. In contrast, P2X3 and P2X4 receptors intensified it. Conclusion P2X2R may mediate ATP-induced LIF expression in astrocytes and multiple ES directly increases LIF expression in astrocytes. Therefore, both ATP/P2X2R and multiple ES-induced increases of LIF expression in astrocytes might mediate the efficacy of ECT on psychiatric disorders. Elucidating detailed mechanisms of ATP/P2X2R and multiple ES-induced LIF expression is expected to result in the identification of new therapeutic targets for psychiatric disorders. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text