Reactivation of latent murine cytomegalovirus from kidney
| Autor: | Diane M. Starnes, Kevin R. Porter, John D. Hamilton |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Male
Kidney Time Factors Salivary gland Congenital cytomegalovirus infection Cytomegalovirus Embryo In Vitro Techniques Biology Virus Replication medicine.disease Virology Virus Mice Tissue culture medicine.anatomical_structure Allogeneic Lymphocyte Nephrology Cytomegalovirus Infections DNA Viral medicine Animals Female Fibroblast |
| Zdroj: | Kidney International. 28(6):922-925 |
| ISSN: | 0085-2538 |
| DOI: | 10.1038/ki.1985.218 |
| Popis: | Reactivation of latent murine cytomegalovirus from kidney. Cytomegalovirus (CMV) is presumed to cause latent infection, but the sites of infection are incompletely known. We propose that latent murine cytomegalovirus is present in kidney and may be reactivated by explantation. Immunosuppressive agents and allogeneic stimuli may enhance this process. Balb/c mice were infected 11 to 14 months previously with 10 5 pfu of Smith strain murine cytomegalovirus intra-peritoneally. Kidneys from 15 infected and nine uninfected mice were washed, minced into 1 to 2mm 2 explants and placed into separate tissue culture wells containing a mouse embryo fibroblast monolayer. Explants were untreated or treated with azathioprine, cyclophosphamide, anti-thymocyte serum, or allogeneic lymphocytes. Daily observations for CPE and passage of supernatant to fresh mouse embryo fibroblast were done. Standard cultures of blood, kidney, and salivary gland were negative. However, virus was isolated from the explants of 8/15 animals, with a reactivation time of 30 to 70 days. No significant difference in reactivation time was noted between treated or untreated expiants. Restriction enzyme analysis of viral DNA confirmed identity with the original strain. These data show that latent murine cytomegalovirus is present in murine kidney tissue and may be reactivated by explantation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|