Paneth Cell Hyperplasia and Metaplasia in Necrotizing Enterocolitis
| Autor: | Ronald R. de Krijger, Maaike W. Schaart, Johannes B. van Goudoever, Patrycja Puiman, Nanda Burger-van Paassen, Adrianus C. J. M. de Bruijn, Dick Tibboel, Ingrid B. Renes |
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| Přispěvatelé: | Pediatrics, Pathology, Pediatric Surgery |
| Rok vydání: | 2011 |
| Předmět: |
Male
Paneth Cells Pathology medicine.medical_specialty Cell Infant Premature Diseases digestive system Enterocolitis Necrotizing Metaplasia medicine Humans Enterocolitis business.industry Infant Newborn Hyperplasia medicine.disease Immunohistochemistry Small intestine digestive system diseases medicine.anatomical_structure Pediatrics Perinatology and Child Health Necrotizing enterocolitis Paneth cell medicine.symptom business Infant Premature Antimicrobial Cationic Peptides |
| Zdroj: | Pediatric Research, 69(3), 217-223. Lippincott Williams & Wilkins |
| ISSN: | 0031-3998 |
| Popis: | Paneth cell dysfunction has been suggested in necrotizing enterocolitis (NEC). The aim of this study was to i) study Paneth cell presence, protein expression, and developmental changes in preterm infants with NEC and ii) determine Paneth cell products and antimicrobial capacity in ileostomy outflow fluid. Intestinal tissue from NEC patients (n = 55), preterm control infants (n = 22), and term controls (n = 7) was obtained during surgical resection and at stoma closure after recovery. Paneth cell abundance and protein expression were analyzed by immunohistochernistry. RNA levels of Paneth cell proteins were determined by real-time quantitative RTPCR. In ileostomy outflow fluid, Paneth cell products were quantified, and antimicrobial activity was measured in vitro. In acute NEC, Paneth cell abundance in small intestinal tissue was not significantly different from preterm controls. After recovery from NEC, Paneth cell hyperplasia was observed in the small intestine concomitant with elevated human alpha-defensin 5 mRNA levels. In the colon, metaplastic Paneth cells were observed. Ileostomy fluid contained Paneth cell proteins and inhibited bacterial growth. In conjunction, these data suggest an important role of Paneth cells and their products in various phases of NEC. (Pediatr Res 69: 217-223, 2011) |
| Databáze: | OpenAIRE |
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