gDNA qPCR is statistically more reliable than mRNA analysis in detecting leukemic cells to monitor CML
| Autor: | Andrea Conti, Anna Michelato, Cristina Barlassina, Giovanni Micheloni, Alessia Rainero, Giorgia Millefanti, Matteo Barcella, Francesca D'Avila, Eleonora Piscitelli, Ksenija Buklijas, Silvia M. Sirchia, Cristina Pirrone, Orietta Spinelli, Emanuela Maserati, Fabrizio Angaroni, R. Casalone, Giovanni Porta, Lucia Tararà, Massimo Caccia, Roberto Valli |
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| Přispěvatelé: | Rainero, A, Angaroni, F, Conti, A, Pirrone, C, Micheloni, G, Tarara, L, Millefanti, G, Maserati, E, Valli, R, Spinelli, O, Buklijas, K, Michelato, A, Casalone, R, Barlassina, C, Barcella, M, Sirchia, S, Piscitelli, E, Caccia, M, Porta, G |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Cancer Research Immunology Fusion Proteins bcr-abl Leukemia Myelogenous Chronic Treatment Outcome Real-Time Polymerase Chain Reaction Article Follow-Up Studie Fusion gene Young Adult 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Cancer stem cell Leukemia Myelogenous Chronic BCR-ABL Positive Precursor B-Cell Lymphoblastic Leukemia-Lymphoma hemic and lymphatic diseases Humans Medicine RNA Messenger lcsh:QH573-671 Protein Kinase Inhibitors Aged Cell Biology Reverse Transcriptase Polymerase Chain Reaction business.industry lcsh:Cytology Reproducibility of Results Myeloid leukemia DNA Middle Aged Protein-Tyrosine Kinases medicine.disease genomic DNA Leukemia Haematopoiesis Treatment Outcome 030104 developmental biology Real-time polymerase chain reaction 030220 oncology & carcinogenesis Imatinib Mesylate Neoplastic Stem Cells Cancer research Female Stem cell business Follow-Up Studies |
| Zdroj: | Cell Death and Disease, Vol 9, Iss 3, Pp 1-8 (2018) Cell Death & Disease |
| ISSN: | 2041-4889 |
| Popis: | Chronic Myeloid Leukemia (CML) is a stem cell cancer that arises when t(9;22) translocation occurs in a hematopoietic stem cells. This event results in the expression of the BCR-ABL1 fusion gene, which codes for a constitutively active tyrosine kinase that is responsible for the transformation of a HSC into a CML stem cell, which then gives rise to a clonal myeloproliferative disease. The introduction of Tyrosine Kinase Inhibitors (TKIs) has revolutionized the management of the disease. However, these drugs do not seem to be able to eradicate the malignancy. Indeed, discontinuation trials (STIM; TWISER; DADI) for those patients who achieved a profound molecular response showed 50% relapsing within 12 months. We performed a comparative analysis on 15 CML patients and one B-ALL patient, between the standard quantitative reverse-transcriptase PCR (qRT–PCR) and our genomic DNA patient-specific quantitative PCR assay (gDNA qPCR). Here we demonstrate that gDNA qPCR is better than standard qRT–PCR in disease monitoring after an average follow-up period of 200 days. Specifically, we statistically demonstrated that DNA negativity is more reliable than RNA negativity in indicating when TKIs therapy can be safely stopped. |
| Databáze: | OpenAIRE |
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