Development of an Organ-on-a-Chip-Device for Study of Placental Pathologies
| Autor: | E. Du, Babak Mosavati, Andrew V. Oleinikov |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Placenta 02 engineering and technology Placental barrier Umbilical vein Diffusion lcsh:Chemistry Pregnancy Lab-On-A-Chip Devices Membrane porosity molecular concentration distribution Maternal-Fetal Exchange lcsh:QH301-705.5 Spectroscopy placenta-on-a-chip Chemistry General Medicine 021001 nanoscience & nanotechnology Trophoblasts Computer Science Applications embryonic structures Female Rheology 0210 nano-technology Porosity Microfluidics microfluidics Models Biological Organ-on-a-chip Article Catalysis Inorganic Chemistry 03 medical and health sciences Fetus Microfluidic channel Human Umbilical Vein Endothelial Cells Humans Physical and Theoretical Chemistry Molecular Biology Organic Chemistry Glucose transporter glucose transport Biological Transport Membranes Artificial Coculture Techniques Kinetics Glucose 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Biophysics |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 8755, p 8755 (2020) International Journal of Molecular Sciences Volume 21 Issue 22 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | The human placenta plays a key role in reproduction and serves as a major interface for maternofetal exchange of nutrients. Study of human placenta pathology presents a great experimental challenge because it is not easily accessible. In this paper, a 3D placenta-on-a-chip model is developed by bioengineering techniques to simulate the placental interface between maternal and fetal blood in vitro. In this model, trophoblasts cells and human umbilical vein endothelial cells are cultured on the opposite sides of a porous polycarbonate membrane, which is sandwiched between two microfluidic channels. Glucose diffusion across this barrier is analyzed under shear flow conditions. Meanwhile, a numerical model of the 3D placenta-on-a-chip model is developed. Numerical results of concentration distributions and the convection&ndash diffusion mass transport is compared to the results obtained from the experiments for validation. Finally, effects of flow rate and membrane porosity on glucose diffusion across the placental barrier are studied using the validated numerical model. The placental model developed here provides a potentially helpful tool to study a variety of other processes at the maternal&ndash fetal interface, for example, effects of drugs or infections like malaria on transport of various substances across the placental barrier. |
| Databáze: | OpenAIRE |
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