Synthesis of oligonucleotides with glucosamine at the 3′-position and evaluation of their biological activity
| Autor: | Yukio Kitade, Takahiro Sugiura, Yoshiaki Kitamura, Remi Nakashima, Xiong Luo |
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| Rok vydání: | 2013 |
| Předmět: |
Exonucleases
Renilla Small interfering RNA Clinical Biochemistry Oligonucleotides Pharmaceutical Science Biochemistry chemistry.chemical_compound Glucosamine RNA interference Drug Discovery Gene silencing Nuclease resistance Animals Humans Transition Temperature RNA Small Interfering Molecular Biology Luciferases Renilla Nuclease biology Oligonucleotide Phosphoric Diester Hydrolases Organic Chemistry Glucosamine analogues RNA Biological activity chemistry siRNA RNAi biology.protein Molecular Medicine RNA Interference HeLa Cells Snake Venoms |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 23(14):4157-4161 |
| ISSN: | 0960-894X |
| DOI: | 10.1016/j.bmcl.2013.05.036 |
| Popis: | Short interfering RNA (siRNA) has been proven to be an utilizable tool for post-transcriptional gene silencing research. In this study, we designed and synthesized two glucosamine analogues and tried to modify the siRNA using these two glucosamine analogues at the 3′-overhang region of siRNAs to improve the nuclease resistance and to overcome some other weak points. The siRNAs modified with glucosamine analogues had almost no effect of the thermal stability and showed strong resistance to nuclease degradation. Some of them kept the same gene silencing activity level as unmodified siRNA. |
| Databáze: | OpenAIRE |
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