LncRNA-MSC-AS1 inhibits the ovarian cancer progression by targeting miR-425-5p
| Autor: | Xinhua Bu, Dandan Zhu, Li Jiang, Yinling Zhao, Ai-hua Huang, Hua Qian, Donglan Yuan, Tianhui Xu, Chiwen Liu |
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| Rok vydání: | 2021 |
| Předmět: |
MicroRNA-425-5p
Proliferation Apoptosis Biology Flow cytometry Ovarian cancer microRNA medicine Humans Cell Proliferation Ovarian Neoplasms Reporter gene medicine.diagnostic_test Cell growth LncRNA MSC-AS1 Research Obstetrics and Gynecology Gynecology and obstetrics Cell cycle medicine.disease MicroRNAs Oncology Cell culture Cancer research RG1-991 Disease Progression Female RNA Long Noncoding |
| Zdroj: | Journal of Ovarian Research Journal of Ovarian Research, Vol 14, Iss 1, Pp 1-11 (2021) |
| ISSN: | 1757-2215 |
| Popis: | Background Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) were reported to be aberrantly expressed and related to the pathogenesis of ovarian cancer. However, the role and regulatory mechanism of MSC-AS1 in ovarian cancer has yet to be fully elucidated. Methods Expression of lncRNA MSC-AS1 (MSC-AS1) and microRNA-425-5p (miR-425-5p) in the ovarian cancer tissue samples and cell lines was examined by quantitative real-time polymerase chain reaction (qRT-PCR). The functions of MSC-AS1 on ovarian cancer cell proliferation, cell cycle and apoptosis were determined using MTT, colony formation and flow cytometry analyses. The protein expression levels were evaluated using western blot assay. The targeting relationship MSC-AS1 and miR-425-5p was verified via dual-luciferase reporter assay. Results MSC-AS1 expression level was lowly expressed, while miR-425-5p level was highly in ovarian cancer tissues and cells. Elevation of MSC-AS1 has the ability to significantly inhibit cell proliferation and facilitate cell apoptosis in SKOV3 and A2780 cells. Moreover, MSC-AS1 targeted and negatively modulated miR-425-5p. MiR-425-5p up-regulation has been proved to partially reverse the tumor suppressive function of MSC-AS1 overexpression Conclusion MSC-AS1 sponged miR-425-5p to inhibit the ovarian cancer progression. These findings may provide a promising therapeutic target for the treatment of ovarian cancer. |
| Databáze: | OpenAIRE |
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