Sialoglycoproteins prepared from the eggs of Carassius auratus prevent bone loss by inhibiting the NF-κB pathway in ovariectomized rats
| Autor: | Jingfeng Wang, Changhu Xue, Yiming Wang, Shanshan Wang, Guanghua Xia, Shu-hong Sun, Yanlei Zhao, Zhe Yu |
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| Rok vydání: | 2016 |
| Předmět: |
Fish Proteins
0301 basic medicine medicine.medical_specialty Ovariectomy Sialoglycoproteins Osteoporosis Osteoclasts Biology Bone resorption Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Osteogenesis Goldfish Internal medicine medicine Animals Humans Ovum TNF Receptor-Associated Factor 6 NF-kappa B Transcription Factor RelA NF-κB General Medicine NFKB1 medicine.disease Rats 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry RANKL Ovariectomized rat biology.protein Female Signal transduction Cancellous bone Signal Transduction Food Science |
| Zdroj: | Food & Function. 7:704-712 |
| ISSN: | 2042-650X 2042-6496 |
| DOI: | 10.1039/c5fo00955c |
| Popis: | In this study, we investigated the improvement of osteoporosis by sialoglycoproteins isolated from the eggs of Carassius auratus (Ca-SGP) in ovariectomized rats. Ca-SGP was supplemented to ovariectomized Sprague-Dawley rats for 90 days. The results showed that Ca-SGP treatment remarkably prevented the reduction of bone mass, improved cancellous bone structure and biochemical properties. Ca-SGP also significantly decreased the serum contents of TRAP, Cath-K, MMP-9, DPD, CTX-1, Ca, and P. Mechanism investigation revealed that Ca-SGP significantly increased the OPG/RANKL ratio in mRNA expression, protein expression and serum content. Further research suggested that NF-κB signaling pathways were inhibited by suppressing the mRNA and protein expressions of NFATc1 and TRAF6, diminishing the mRNA expression and phosphorylation of NF-κB p65, three key transcription factors in NF-κB pathways. These results suggest that Ca-SGP can improve osteoporosis by inhibiting bone resorption via suppressing the activation of osteoclastogenesis related NF-κB pathways. |
| Databáze: | OpenAIRE |
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